APEX™ Antibody Labeling Kits

APEX® Antibody Labeling Kits utilize a unique solid-phase labeling method that enables an efficient, convenient and reliable method for covalently attaching fluorescent labels to very small amounts of IgG antibody in the presence of any contaminants including stabilizing proteins and amine-containing buffers.

  • Reliability—Produces conjugates with stable, covalent attachment of superior fluorescent labels
  • Convenience—Requires only 10–20 µg of IgG antibody per labeling reaction, regardless of any purity limitations
  • Complete—Includes everything required to perform 5 separate labelings

How APEX® Antibody Labeling Kits work

The APEX® Antibody Labeling Kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX® antibody labeling tip (Figure 1). Any contaminants, including stabilizing proteins or amine-containing buffers, are simply eluted through the tip. After applying the amine-reactive label, a fluorescent IgG conjugate is ready for use in any imaging or flow cytometry application in as little as 2.5 hours with very little hands-on time. The typical yield of labeled antibody using this method ranges from 40–80%.

The APEX® Antibody Labeling Kits includes all reagents required to perform 5 separate labeling reactions of 10–20 μg of IgG antibody with one of Molecular Probes® superior fluorophores or biotin-XX. The biotin-XX provides a 14-atom spacer between the antibody and the biotin moiety.

   APEX™ Antibody Labeling Kits
Figure 1. APEX® antibody labeling tip.		  

APEX® Antibody Labeling Kits selection guide

Fluorophore or biotin label Ex/Em (nm) Application Cat. No.
Alexa Fluor® 488 496/519 Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti–Alexa Fluor® 488 antibodies. A10468
Alexa Fluor® 555 555/565 Fluorescent label for use in imaging A10470
Alexa Fluor® 568 578/603 A10494
Alexa Fluor® 594 590/617 A10474
Alexa Fluor® 647 650/665 Fluorescent label for use in imaging or flow cytometry A10475
Oregon Green® 488 496/524 Fluorescent label for use in imaging or flow cytometry; hapten for signal amplification with anti-fluorescein/Oregon Green® antibodies A10476
Pacific Blue™ dye 416/451 Fluorescent label for use in imaging or flow cytometry A10478
Biotin-XX NA Streptavidin, avidin, and NeutrAvidin® binding partner for use in imaging or flow cytometry A10495
Ex/Em = Fluorescence excitation and emission maxima, in nm.

Need to label other antibody amounts?

IgG amount Attachment of label Products
<1–20 µg Site-specific Zenon® Antibody Labeling Kits
<100 µg Site-specific SiteClick™ Antibody Labeling Kits
10–20 µg Conventional covalent APEX® Antibody Labeling Kits
20–100 µg Conventional covalent Microscale Protein Labeling Kits
100 µg Conventional covalent Antibody Labeling Kits
1 mg Conventional covalent Protein Labeling Kits
0.5–3 mg Conventional covalent SAIVI™ Rapid Antibody Labeling Kits

Resources

Research tools

BioProbes® Journal articles

Molecular Probes® Handbook

For Research Use Only. Not for use in diagnostic procedures.