GeneArt Site-Directed Mutagenesis Service

Site-directed mutagenesis is a molecular biology technique that is used to introduce specific changes at desired locations within a DNA fragment; with this technique, complete de novo synthesis is no longer required. Mutagenesis of existing genes can help you find answers for your research, including:

• Investigating active sites, structure-function relationships, nucleic acid–protein interactions, etc.
• Testing functional elements (promoter regions, terminator sequences, etc.)
• Constructing fusion proteins and tagged proteins
• Designing knockout constructs
• Generating alternative splice forms
• Conducting alanine scans to identify protein functional domains

The GeneArt site-directed mutagenesis service introduces single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequences, quickly and economically. We use a targeted PCR-based process to introduce genetic variation precisely where you need it and then clone the construct into the vector of your choice. All final sequences are 100% sequence-verified and documented to help ensure that the DNA you receive is exactly what you’ve requested.

Service features

• Mutations exactly where you need them, without the risk of introducing unwanted mutations
• Unlike “quick change” strategies, the vector is not subject to PCR—just the insert
• Insert size can be as large as 6–8 kb
• No mutations in the vector backbone
• Full sequence verification of insert DNA
• Mutations can be clustered or far apart, with the same cost and production time
• Template constructs are stored for at least 2 years at no charge, and longer-term storage is available
• Competitive pricing and fast turnaround times

Download the Multiple Sequence Submission Form, enter your DNA sequence request(s) using Microsoft Excel software, and email it to our customer support team at geneartsupport@thermofisher.com.

For further information regarding this service or the order process, please contact geneartsupport@thermofisher.com

For your convenience, GeneArt mutagenesis services offers precise and customizable variant creation with guaranteed sequence verification and efficient processing times.