Site-directed mutagenesis techniques allow specific introduction of variations at desired locations within DNA, without the need for complete de novo DNA synthesis. Mutagenesis of existing genes can help you find answers for your research, such as:
- Investigate active sites, structure-function relationships, nucleic acid–protein interactions, etc.
- Test functional elements (promoter regions, terminator sequences, etc.)
- Construct fusion proteins and tagged proteins
- Design knockout constructs
- Generate alternative splice forms
- Conduct alanine scans to identify protein functional domains