Description

TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.

  • Highly specific – quantitiate miRNA transcription from a single genomic locus
  • Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
  • A new perspective – measure miRNA expression at the gene level

TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and Selection 
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop.

sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

Catalog # Size # of Reactions/Concentration
4427012 Small 360/20X
4427013 Medium 750/20X
4427014 Large 2900/60X

Specifications

General Specifications

Pre-formulated assay (20x or 60x mix):
  • 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration
  • 1 FAM™ dye-labeled TaqMan® MGB probe (250 nM final 1X reaction concentration)
Documentation: On compact disc:
  • MSDS Formamide
  • MSDS Non-formamide
  • Protocol
  • Quick Reference Card
  • Understanding your Shipment
  • Assay Info File
  • Certificate of Analysis
  • Data Sheet
Printed copies of above documents
Assay Information File: Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context sequence, Amplicon size)
Tracking/Identification:
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes

 

Shipping and Storage

Shipped at ambient temperature. 
Store at –15ºC to –25ºC. 

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Documents

Manuals & Protocols

Product Literature

Help Center

Order Support

Contact Order & Web Support, Search FAQs, View Order History and Track Your Order

Technical Support

Contact a Technical Application Scientist, Search Product Documents & FAQs and Instrument Maintenance & Repair Requests

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Description

TaqMan® Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the most upstream RNA molecules in the microRNA biogenesis pathway. They are designed using our state-of-the-art bioinformatics pipeline, delivering gold standard assay performance and data quality for pri-miRNA studies.

  • Highly specific – quantitiate miRNA transcription from a single genomic locus
  • Fast, simple, and scalable – two-step RT-qPCR provides high-quality results
  • A new perspective – measure miRNA expression at the gene level

TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic Assays are renowned, to the detection of pri-miRNA transcripts. As a result of their high sensitivity, TaqMan® Pri-miRNA Assays require as little as 1 ng of sample input to quantify expression from moderate- to high-expression miRNA loci.

Design and Selection
Since all pri-miRNA transcripts have not been mapped, these assays are designed within close proximity to each stem-loop sequence represented in the Sanger miRBase sequence repository. This ensures accurate measurement of the transcript of interest. Each publicly available stem-loop can be matched to a TaqMan® Pri-miRNA Assay and TaqMan® MicroRNA Assay, enabling the RNA sequences produced at either end of the miRNA maturation pathway to be quantified independently.

This product can be purchased by visiting AppliedBiosystems.com.
Direct ordering through the Life Technologies website will be available soon.

Specifications

General Specifications

Pre-formulated assay (20x or 60x mix):
  • 2 unlabeled PCR primers (900 nM each final 1X reaction concentration; 150 nM final 1X reaction concentration
  • 1 FAM™ dye-labeled TaqMan® MGB probe (250 nM final 1X reaction concentration)
Documentation:On compact disc:
  • MSDS Formamide
  • MSDS Non-formamide
  • Protocol
  • Quick Reference Card
  • Understanding your Shipment
  • Assay Info File
  • Certificate of Analysis
  • Data Sheet
Printed copies of above documents
Assay Information File:Includes Sales Order Information, List of Contents (Assay ID, Part Number, Expiration Date, & Lot Number), Annotation Information (Gene Symbol, Gene Name, Entrez Gene ID, Transcript Accession & Location on Transcript) and Design Details (Context sequence, Amplicon size)
Tracking/Identification:
  • 2D barcode laser-etched on the bottom of each assay tube
  • 1D barcode printed on each rack of tubes

Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Help Center

Order Support

Contact Order & Web Support, Search FAQs, View Order History and Track Your Order

Technical Support

Contact a Technical Application Scientist, Search Product Documents & FAQs and Instrument Maintenance & Repair Requests

Support Tools