Invitrogen Power Blotter

Versatile semi-dry blotting – the performance you want with the flexibility you need

The Invitrogen Power Blotter is our flexible solution for western blot transfer, from interchangeable blotting cassettes to suit your required throughput, to multiple transfer stack consumable choices.

Designed for rapid 5-10 minute semi-dry transfer of proteins from polyacrylamide gels to nitrocellulose or PVDF membranes, the Invitrogen Power Blotter utilizes an integrated power supply, LCD touchscreen, and pre-programmed, optimized transfer protocols.

Harness the power of the Invitrogen Power Blotter in your western blotting workflow. Explore more in the tabs below.

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The Invitrogen Power Blotter is an all-in-one, integrated transfer system. There is no need for an external power supply – the Power Blotter features an integrated power supply capable of delivering high current for rapid transfer. Select from a series of pre-programmed, optimized transfer methods on the LCD touchscreen depending on your protein(s) of interest, or create custom programs based on your needs.

User-friendly
Transfer method Constant amperage Default run time
Low MW (<25 kDa) 1.3 Amps 5 minutes
Mixed-range MW (25-150 kDa) 1.3 Amps 7 minutes
High MW (>150 kDa) 1.3 Amps 10 minutes
1.5 mm thick gels 1.3 Amps 10 minutes
  Constant voltage Default run time
Standard semi-dry (with Towbin Transfer Buffer) 25 volts (1.0 Amp) 60 minutes

The Invitrogen Power Blotter is our most flexible transfer device – offering options based on throughput and transfer speed, making Power Blotter the transfer device suited to grow around your lab’s needs.

The Power Blotter Cassette allows for simultaneous transfer of 1 to 2 mini-sized gels or 1 midi-sized gel. The Power Blotter XL Cassette provides the potential for increased throughput, for simultaneous transfer of up to 4 mini-sized gels or up to 2 midi-sized gels. Both cassettes are interchangable with the Power Blotter Station power supply and control base to form the Power Blotter System or Power Blotter XL System.

Power Blotter
Power Blotter
Transfer 1-2 mini-sized gels or 1 midi-sized gel
Power Blotter
Power Blotter
Power Blotter
Transfer 1-4 mini-sized gels or 2 midi-sized gels
Power Blotter XL

The Invitrogen Power Blotter can be used with our do-it-yourself or ready-to-use transfer stacks.

The Invitrogen Power Blotter Pre-cut Membranes and Filters come prepared with your choice of nitrocellulose (0.2 and 0.45 uM pore sizes available) or PVDF (0.45 uM pore size available) sandwiched between four sheets of our 0.85 mm thick filter papers, which combine for the appropriate thickness and buffer capacity for optimal performance. Pairing the Power Blotter Pre-cut Membranes and Filters with the Power Blotter 1-Step Transfer Buffer allow for high efficiency semi-dry transfer of proteins in as little as 5 minutes. The Power Blotter 1-Step Transfer Buffer is a specialized high ionic strength transfer buffer, which allows for the increased current necessary to deliver rapid transfer.

Diagram of Power Blotter cassette with Power Blotter Pre-Cut Membrane and Filters

Diagram of Power Blotter cassette with Power Blotter Pre-Cut Membrane and Filters. (1) Cathode, (2) 2 sheets of filter paper, (3) Pre-run gel, (4) Membrane, (5) 2 sheets of filter paper, (6) Anode.

Membranes and Filters

Power Blotter Pre-Cut Membranes and Filters.

As an alternative, more convenient option, the Power Blotter Select Stacks are pre-assembled transfer stacks that utilize a gel matrix technology which incorporates optimized anode and cathode buffers to serve as ion reservoirs necessary for transfer. These gel matrix buffer reservoirs enable the system to generate a high field strength to increases the overall transfer speed and efficiency. Additionally, the gel matrix format of the Power Blotter Select Transfer Stacks eliminates the need for preparing buffers, soaking filter paper and membranes, and minimizes handling that can lead to inconsistent performance.

Diagram of Power Blotter cassette with Power Blotter Select Stacks

Diagram of Power Blotter cassette with Power Blotter Select Stacks. (1) Cathode, (2) Cathode gel matrix with protective sheet above, (3) Pre-run gel, (4) Membrane, (5) Anode gel matrix with protective sheet underneath, (6) Anode.

Power Blotter Select Transfer Stacks.

Power Blotter Select Transfer Stacks.

 

The Invitrogen Power Blotter offers high transfer efficiency for a broad range of protein sizes.

Apparatus and consumables Power Blotter with Select Transfer Stacks Power Blotter with Pre-cut Membranes and Filters and Power Blotter 1-Step Transfer Buffer
Transfer time 5-10 minutes 5-10 minutes
Transfer efficiency
Power Blotter Select Transfer Stacks and Power Blotter Pre-Cut Membrane and Filter stacks efficiently transfer high, mid, and low molecular weight proteins. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and Cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 µg HeLa lysate, 7.5 µg KLH spike per well, serially diluted 2:3) onto Bolt 4-12% Bis-Tris Plus gels. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310; PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320; PB7320), probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. Images captured using automatic exposure on an iBright FL1000 Imaging System.

Power Blotter delivers reliable results. For increased reproducibility, Power Blotter Select Transfer Stacks deliver exceptional performance.

12 individual Invitrogen Bolt 4-12% Bis-Tris Plus gels transferred using Invitrogen Power Blotter with Invitrogen Power Blotter Select Transfer Stacks

Reliable blot to blot transfer performance. 12 individual Bolt 4-12% Bis-Tris Plus gels transferred using Power Blotter with Power Blotter Select Transfer Stacks. Each blot probed for the same target proteins.

The handling inconsistencies that can occur with a more traditional do-it-yourself transfer stack setup include uneven hydration of membranes, insufficient removal of methanol/ethanol from activated PVDF membrane, incorrect setup of stack (too many and/or too few filter paper layers, incorrect membrane and/or gel orientation), drying of stacks while others are being assembled, and contaminated buffers, all of which can lead to less than ideal transfer performance.

Power Blotter Select Transfer Stacks help eliminate handling inconsistencies that can affect transfer performance

Power Blotter Select Transfer Stacks help eliminate handling inconsistencies that can affect transfer performance. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and p23 protein) was performed by loading serially diluted HeLa cell lysate starting at 7.5 µg to 200 ng (2 to 3 dilution) per well spiked with serially diluted KLH protein starting at 7.5 ng to 0.20 ng per well onto Bolt 4-12% Bis-Tris Plus gels. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310; PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320; PB7320), probed with target-specific primary antibodies and HRP conjugated secondary antibodies, and incubated with SuperSignal West Dura Extended Duration Substrate. Images captured using automatic exposure on an iBright FL1000 Imaging System.

Get a jumpstart and save money with our welcome packs!

Our welcome packs include everything you need to get started transferring your proteins with the Invitrogen Power Blotter.

Option 1

Just getting started?

Choose our Power Blotter Welcome Pack which includes:

  • Power Blotter Station
  • Power Blotter Cassette
  • Power Blotter Select Transfer Stacks, Nitrocellulose, Regular size
  • Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular size
  • Power Blotter 1-Step Transfer Buffer
Power Blotter Welcome Pack

Power Blotter Welcome Pack

Option 2

Need more throughput?

Choose our Power Blotter XL Welcome Pack which includes:

  • Power Blotter Station
  • Power Blotter XL Cassette
  • Power Blotter Select Transfer Stacks, Nitrocellulose, Regular size
  • Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular size
  • Power Blotter 1-Step Transfer Buffer
Power Blotter XL Welcome Pack

Power Blotter XL Welcome Pack

The Invitrogen Power Blotter is an all-in-one, integrated transfer system. There is no need for an external power supply – the Power Blotter features an integrated power supply capable of delivering high current for rapid transfer. Select from a series of pre-programmed, optimized transfer methods on the LCD touchscreen depending on your protein(s) of interest, or create custom programs based on your needs.

User-friendly
Transfer method Constant amperage Default run time
Low MW (<25 kDa) 1.3 Amps 5 minutes
Mixed-range MW (25-150 kDa) 1.3 Amps 7 minutes
High MW (>150 kDa) 1.3 Amps 10 minutes
1.5 mm thick gels 1.3 Amps 10 minutes
  Constant voltage Default run time
Standard semi-dry (with Towbin Transfer Buffer) 25 volts (1.0 Amp) 60 minutes

The Invitrogen Power Blotter is our most flexible transfer device – offering options based on throughput and transfer speed, making Power Blotter the transfer device suited to grow around your lab’s needs.

The Power Blotter Cassette allows for simultaneous transfer of 1 to 2 mini-sized gels or 1 midi-sized gel. The Power Blotter XL Cassette provides the potential for increased throughput, for simultaneous transfer of up to 4 mini-sized gels or up to 2 midi-sized gels. Both cassettes are interchangable with the Power Blotter Station power supply and control base to form the Power Blotter System or Power Blotter XL System.

Power Blotter
Power Blotter
Transfer 1-2 mini-sized gels or 1 midi-sized gel
Power Blotter
Power Blotter
Power Blotter
Transfer 1-4 mini-sized gels or 2 midi-sized gels
Power Blotter XL

The Invitrogen Power Blotter can be used with our do-it-yourself or ready-to-use transfer stacks.

The Invitrogen Power Blotter Pre-cut Membranes and Filters come prepared with your choice of nitrocellulose (0.2 and 0.45 uM pore sizes available) or PVDF (0.45 uM pore size available) sandwiched between four sheets of our 0.85 mm thick filter papers, which combine for the appropriate thickness and buffer capacity for optimal performance. Pairing the Power Blotter Pre-cut Membranes and Filters with the Power Blotter 1-Step Transfer Buffer allow for high efficiency semi-dry transfer of proteins in as little as 5 minutes. The Power Blotter 1-Step Transfer Buffer is a specialized high ionic strength transfer buffer, which allows for the increased current necessary to deliver rapid transfer.

Diagram of Power Blotter cassette with Power Blotter Pre-Cut Membrane and Filters

Diagram of Power Blotter cassette with Power Blotter Pre-Cut Membrane and Filters. (1) Cathode, (2) 2 sheets of filter paper, (3) Pre-run gel, (4) Membrane, (5) 2 sheets of filter paper, (6) Anode.

Membranes and Filters

Power Blotter Pre-Cut Membranes and Filters.

As an alternative, more convenient option, the Power Blotter Select Stacks are pre-assembled transfer stacks that utilize a gel matrix technology which incorporates optimized anode and cathode buffers to serve as ion reservoirs necessary for transfer. These gel matrix buffer reservoirs enable the system to generate a high field strength to increases the overall transfer speed and efficiency. Additionally, the gel matrix format of the Power Blotter Select Transfer Stacks eliminates the need for preparing buffers, soaking filter paper and membranes, and minimizes handling that can lead to inconsistent performance.

Diagram of Power Blotter cassette with Power Blotter Select Stacks

Diagram of Power Blotter cassette with Power Blotter Select Stacks. (1) Cathode, (2) Cathode gel matrix with protective sheet above, (3) Pre-run gel, (4) Membrane, (5) Anode gel matrix with protective sheet underneath, (6) Anode.

Power Blotter Select Transfer Stacks.

Power Blotter Select Transfer Stacks.

 

The Invitrogen Power Blotter offers high transfer efficiency for a broad range of protein sizes.

Apparatus and consumables Power Blotter with Select Transfer Stacks Power Blotter with Pre-cut Membranes and Filters and Power Blotter 1-Step Transfer Buffer
Transfer time 5-10 minutes 5-10 minutes
Transfer efficiency
Power Blotter Select Transfer Stacks and Power Blotter Pre-Cut Membrane and Filter stacks efficiently transfer high, mid, and low molecular weight proteins. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and Cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 µg HeLa lysate, 7.5 µg KLH spike per well, serially diluted 2:3) onto Bolt 4-12% Bis-Tris Plus gels. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310; PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320; PB7320), probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. Images captured using automatic exposure on an iBright FL1000 Imaging System.

Power Blotter delivers reliable results. For increased reproducibility, Power Blotter Select Transfer Stacks deliver exceptional performance.

12 individual Invitrogen Bolt 4-12% Bis-Tris Plus gels transferred using Invitrogen Power Blotter with Invitrogen Power Blotter Select Transfer Stacks

Reliable blot to blot transfer performance. 12 individual Bolt 4-12% Bis-Tris Plus gels transferred using Power Blotter with Power Blotter Select Transfer Stacks. Each blot probed for the same target proteins.

The handling inconsistencies that can occur with a more traditional do-it-yourself transfer stack setup include uneven hydration of membranes, insufficient removal of methanol/ethanol from activated PVDF membrane, incorrect setup of stack (too many and/or too few filter paper layers, incorrect membrane and/or gel orientation), drying of stacks while others are being assembled, and contaminated buffers, all of which can lead to less than ideal transfer performance.

Power Blotter Select Transfer Stacks help eliminate handling inconsistencies that can affect transfer performance

Power Blotter Select Transfer Stacks help eliminate handling inconsistencies that can affect transfer performance. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and p23 protein) was performed by loading serially diluted HeLa cell lysate starting at 7.5 µg to 200 ng (2 to 3 dilution) per well spiked with serially diluted KLH protein starting at 7.5 ng to 0.20 ng per well onto Bolt 4-12% Bis-Tris Plus gels. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310; PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320; PB7320), probed with target-specific primary antibodies and HRP conjugated secondary antibodies, and incubated with SuperSignal West Dura Extended Duration Substrate. Images captured using automatic exposure on an iBright FL1000 Imaging System.

Get a jumpstart and save money with our welcome packs!

Our welcome packs include everything you need to get started transferring your proteins with the Invitrogen Power Blotter.

Option 1

Just getting started?

Choose our Power Blotter Welcome Pack which includes:

  • Power Blotter Station
  • Power Blotter Cassette
  • Power Blotter Select Transfer Stacks, Nitrocellulose, Regular size
  • Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular size
  • Power Blotter 1-Step Transfer Buffer
Power Blotter Welcome Pack

Power Blotter Welcome Pack

Option 2

Need more throughput?

Choose our Power Blotter XL Welcome Pack which includes:

  • Power Blotter Station
  • Power Blotter XL Cassette
  • Power Blotter Select Transfer Stacks, Nitrocellulose, Regular size
  • Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular size
  • Power Blotter 1-Step Transfer Buffer
Power Blotter XL Welcome Pack

Power Blotter XL Welcome Pack

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