Sequence specific proteases are utilized to cleave proteins into smaller fragments, or peptides. Trypsin is the protease of choice for protein digestion. However, separate or sequential digestion with alternative proteases, such as Glu-C, LysN, Lys-C, Asp-N or Chymotrypsin, can improve individual protein sequence coverage or generate unique peptide sequences for different MS applications. The Thermo Scientific Pierce Proteases are highly purified and modified for optimal protein digestion and validated for use in MS. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high quality samples that are ready for MS analysis in less than 3 hours.

  • Comprehensive—broad range of proteases to accommodate a variety of digestion strategies
  • Convenient —complete in-gel and in-solution kits are available as well as standalone proteases in multiple pack sizes
  • Economical—bulk and custom packaging of some proteases provide more cost effective or optimized options
  • Validated—all proteases have been fully tested and processed samples have been analyzed using Thermo Scientific Mass Spectrometers

Choose the right protease for your application and cleavage site

 
  Pierce Trypsin Protease, MS Grade Pierce LysN Protease, MS Grade Pierce Trypsin/Lys-C Protease Mix, MS-Grade LysC Protease, MS Grade
Source Porcine pancreatic extracts Grifola frondosa Porcine pancreatic extracts, Lysobacter enzymogenes Lysobacter enzymogenes
Cleavage specificity Carboxyl side of arginine and lysine residues Amino side of lysine residues Carboxyl side of arginine and lysine residues Carboxyl side of lysine residues
Modified? Yes, TPCK-treated and methylated No Yes (trypsin); No (LysC) No
Formats Lyophilized or Frozen Lyophilized Lyophilized Lyophilized
Standard packaging 20 or 100 µg/vial 20 µg/vial 20 or 100 µg/vial 20 or 100 µg/vial
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  Chymotrypsin Glu-C Protease, MS Grade Asp-N Protease, MS Grade
Source Bovine pancreatic extracts Staphylococcus aureus Pseudomonas fragi
Cleavage specificity Carboxyl side of tyrosine, phenylalanine, tryptophan and leucine Carboxyl side of glutamate Amino side of aspartate residues
Modified? Yes, TLCK-treated No No
Formats Lyophilized Lyophilized Lyophilized
Standard packaging 4 x 25 µg/vial 10 µg/vial 2 µg/vial
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  Pierce Trypsin Protease, MS Grade Pierce LysN Protease, MS Grade Pierce Trypsin/Lys-C Protease Mix, MS-Grade LysC Protease, MS Grade
Source Porcine pancreatic extracts Grifola frondosa Porcine pancreatic extracts, Lysobacter enzymogenes Lysobacter enzymogenes
Cleavage specificity Carboxyl side of arginine and lysine residues Amino side of lysine residues Carboxyl side of arginine and lysine residues Carboxyl side of lysine residues
Modified? Yes, TPCK-treated and methylated No Yes (trypsin); No (LysC) No
Formats Lyophilized or Frozen Lyophilized Lyophilized Lyophilized
Standard packaging 20 or 100 µg/vial 20 µg/vial 20 or 100 µg/vial 20 or 100 µg/vial
  Order now Order now Order now Order now
 
  Chymotrypsin Glu-C Protease, MS Grade Asp-N Protease, MS Grade
Source Bovine pancreatic extracts Staphylococcus aureus Pseudomonas fragi
Cleavage specificity Carboxyl side of tyrosine, phenylalanine, tryptophan and leucine Carboxyl side of glutamate Amino side of aspartate residues
Modified? Yes, TLCK-treated No No
Formats Lyophilized Lyophilized Lyophilized
Standard packaging 4 x 25 µg/vial 10 µg/vial 2 µg/vial
  Order now Order now Order now

Trypsin/Lys C Mix data

Quality of peptide samples achieved using Pierce trypsin/Lys-C mix

Quality of peptide samples achieved using Pierce Trypsin/Lys-C mix is as good as or better than protein samples prepared using another supplier’s trypsin/Lys-C mix.


In-gel or in-solution tryptic digestion?

The decision to perform in-solution or in-gel digestion is generally determined by the sample amount and/or its complexity.

In-solution digestion is recommended for small sample volumes with low to moderate complexity and is more amenable to high-throughput sample.

For highly complex samples, in-gel digestion has an inherent advantage because the SDS-PAGE workflow combines protein denaturation with separation, and provides a visual indication of the relative protein abundance in the sample,  although peptide recovery is reduced.

We offer complete kits for both in-gel and in-solution sample processing. 


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Featured data

Protein LysN Trypsin Merged
Moesin 21% 24% 37%
Src substrate cortactin 25% 10% 30%
Glutamate dehydrogenase 1 16% 12% 28%
Epoxide hydrolase 1 13% 14% 23%

Percent sequence coverage of selected proteins in an A549 cell lysate upon in-solution digestion with LysN protease or trypsin. The total (merged) sequence coverage represents the combined coverage of separate LysN and trypsin digestions followed by LC-MS/MS analysis with CID.