PSC research requires careful attention to culture conditions. From KnockOut media to Essential 8 media and cell therapy research media, Gibco media deliver culture with confidence. Find the right solution for your stem cell culture needs.

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Which pluripotent stem cell culture medium is right for you?

  Feeder-Dependent culture Feeder-Free Culture


KnockOut Serum Replacement – MultiSpecies + DMEM F-12 or KnockOut DMEM

CTS KnockOut SR XenoFree Medium* + CTS KnockOut DMEM

StemFlex Medium

Essential 8 Media

Ideal for

Feeder-based human and mouse PSC culture, reprogramming, gene editing, and differentiation

Translational or clinical research applications

Robust maintenance of PSC culture, difficult cell lines, and superior performance in single cell passaging and gene editing

Translational or clinical research applications, researchers needing to control what is in their media system, superior reprogramming

Recommended cell types

Mammalian PSCs

Human PSCs

Human PSCs

Human PSCs

XenoFree formulation





Weekend-free feeding schedule




Essential 8 Flex Medium

Recommended  matrix

Gibco Mouse Embryonic Fibroblasts and Attachment Factor

Gibco CTS CELLstar Substrate**

Geltrex Matrix

Gibco Vitronectin (VTN-N) Recombinant Human Protein

Other compatible matrices



Gibco Vitronectin (VTN-N) Recombinant Human Protein or
rhLaminin 521


Recommended level of dissociation and passaging reagent


Gibco Collagenase IV

Versene Solution

Versene Solution

Versene Solution

Small cluster



StemPro Accutase™ Cell Dissociation Reagent


Single cell

(mouse) – TrypLE Select  Enzyme

Gibco CTS TrypLE Select Enzyme**

TrypLE Select Enzyme

RevitaCell Supplement with Essential 8 Flex Medium

Additional reagents

KnockOut DMEM (for mouse PSC), LIF or bFGF, GlutaMAX-I, NEAA, 2-mercaptoethanol   

bFGF CTS and 2-mercaptoethanol

RevitaCell Supplement optional for added boost and support

RevitaCell Supplement suggested for stressful events

Unless otherwise indicated, all products are For Research Use Only. Not for use in diagnostic procedures
*For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal Law restricts this device to sale by or on the order of a physician.
**For Research Use or Noncommercial Manufacturing of Cell-Based Products for Clinical Research. CAUTION: Not for direct administration into humans or animals.