When mAb Molecules Misbehave

Taming misbehaving mAbs starts with the right purification strategies

The key to taming them is finding the right purification strategies and chromatography resins.

Purification challenges | Explore solutions by challenge | View resources

It’s chromatography chaos

Not all mAbs play nicely. Watch chromatography chaos unfold, then explore how Thermo Fisher Scientific chromatography resins can help restore order with solutions designed for a range of antibody formats, helping streamline workflows, improve yield, and support greater productivity.

Do these purification challenges sound familiar?

When novel mAbs refuse to follow the rules, conventional chromatography strategies can struggle to keep up. Download this article to explore approaches designed to help overcome key purification challenges.

Challenging impurity removal

Limited Protein A selectivity

Increased aggregation risk

More downstream processing

Solutions for common mAb purification challenges

Protein A limitations	When traditional Protein A affinity resins don't provide sufficient binding, selectivity, or recovery, explore alternative affinity ligands and mixed-mode chromatography for difficult-to-purify mAbs.
High aggregates	Reduce aggregates while maintaining product quality by combining affinity capture with hydrophobic interaction (HIC) and mixed-mode chromatography for high-resolution impurity removal.
Novel mAb formats	Purify bispecifics, fragments, Fc-fusion proteins, and other complex modalities with specialized affinity resins designed for emerging therapeutics.
Charge variants	Create a purification strategy to improve separation of acidic and basic charge variants after affinity capture using cation exchange (CEX) and anion exchange (AEX) chromatography to achieve higher purity.
Low recovery	Increase recovery of difficult mAbs with our Protein A or affinity capture conditions and complementary chromatography resins that balance purity with product yield.
Not sure where to start?	Complex mAbs often present unique purification challenges. Speak with a Thermo Fisher purification specialist to develop an affinity capture and downstream purification strategy tailored to your molecule.

Resources for a mAb, mAb world

Address 5 key purification challenges in complex mAbs

The added structural complexity of novel mAbs can introduce purification challenges that conventional chromatography strategies may not fully address. Explore approaches to help overcome key purification challenges.

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Beyond Protein A: Efficient purification strategies for complex mAbs

As modern antibody formats grow more complex, traditional protein A–based processes can become a limiting step. Explore innovative approaches that leverage subdomain-specific ligands to help address purification challenges.

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Improve mAb purification productivity

Explore Thermo Scientific affinity chromatography resins that help enable efficient capture of therapeutic mAbs across a range of feed streams, while helping reduce process steps and improve yield to support purification productivity.

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Why should you evaluate our resins?

Our resins are:

Built for challenging mAb purification
Offer alternative purification strategies for protein A and beyond

Offer scalable solutions to speed process development
Designed with high selectivity for difficult impurity removal

Not sure which resins are right for you?

Selecting the right resin depends on your molecule type, process stage, and purification goals. Compare chemistries, binding capacities, and scalability data in one place.

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