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Back to Basics Series – HPLC
Webinar
Sep 27, 2023
In this “Back to Basics” series for high performance liquid chromatography (HPLC), we cater to both seasoned chromatographers and those freshly embarking on their LC endeavors. This comprehensive series begins with an overview of the principles of HPLC, before diving into column selection and detector selection between optical, charged aerosol detectors and mass detectors.
For advanced chromatographers, understand how to develop and transfer an analytical method and perform system maintenance. Finally, we have tips and tricks covering best practices of working with LC instrumentation. We invite you to join us and take advantage of this opportunity to enhance your understanding of LC.
Sessions:
Tips and tricks of HPLC
Dr. Susanne Fabel | HPLC Product Marketing Manager, Thermo Fisher Scientific
Mr. Sumit Verma | Regional Program Manager, Thermo Fisher Scientific
The webinar is for novice and experienced LC user, who wants to refresh their knowledge on the best practices of working with LC instrumentation.
Join Thermo Fisher Scientific experts on the flow path journey from sample injection to detection to learn useful tips on:
- Learn what might impact your chromatographic separation and detection, before you start your work.
- How to choose the right configuration and settings to achieve the best results.
- How to keep your system healthy and running.
By attending this webinar, you will learn to optimize your HPLC and UHPLC systems operations to achieve the best results for your LC separation.
Dr. Susanne Fabel is a Product Marketing Manager in the Chromatography and Mass Spectrometry group at Thermo Fisher Scientific. Her passion is to help develop world-class HPLC instruments to take science one step further. She has more than 15 years of experience in chromatography and holds a PhD in analytical chemistry from the Technical University of Munich.
Sumit Verma, is a distinguished Product Market Manager in the Asia-Pacific and Japan region. He is deeply associated with Chromatography and Mass spectrometry at Thermo Fisher Scientific, where he has amassed 18 years of rich experience in the Analytical Industry. Armed with a Post Graduation in Electronics and Management studies from IIMA India, Sumit embarked on his professional journey as a service engineer before venturing into Sales. Now, as a Product Marketing expert, he thrives on collaborating closely with customers to drive product improvement and innovation, aiming to simplify and enrich their lives.
Principles of HPLC
A basic introduction and overview of HPLC and the fundamental theories covering the following topics:
- What is HPLC?
- How a HPLC works
- Basic theory of separation
- Introduction to column chemistry
Learning Points:
- HPLC theory
- Basic column chemistry
- Hardware principles
Who should attend:
- Users new to HPLC
- Users who want a refresher
HPLC theory overview
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
A journey through a HPLC
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
Life isn’t all C18 - retaining tricky compounds
Looking at the alternatives to the standard approach of using C18 based phase. An opportunity more about some of the more unique column phases and how to optimize your chromatography.
Learning Points:
- Column chemistry
- Retaining problematic compounds
- Optimal conditions for unique phases
Who should attend:
- New to HPLC
- Users who want a refresher
Life beyond C18 - retaining tricky analytes
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Biopharma separation - large molecule analysis
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Method development using different HPLC column selectivities
Petra Gerhards | Regional Marketing Manager – Chromatography Consumables, Thermo Fisher Scientific
Petra´s background is over 30 years of experience in the field of GCMS, SPE and LCMS. Since joining the EMEA Regional Marketing Team for CCS she has been involved in a lot of workflow solutions combining Vials and Closures with SPE solutions, GCMS and LCMS. She works with KOL`s on data for regional specific marketing campaigns, organizes inhouse seminars and works on customer specific solutions. Her main expertise is in the field of Doping and Drugs of Abuse analysis.
Getting the most out of your HPLC – method development and transfer
The first part of the session will be focused on looking at method development covering the following topics:
- Using the right detector for your compounds
- Method optimization
- Choosing the right column
The second part of the session will be focused on method transfer covering the following topics:
- Transfer between instruments
- Method speed-up
- Important considerations to take on board when transferring methods
Learning Points:
- Method development
- Method transfer
- Introduction to UHPLC
Who should attend:
- Users new to HPLC
- Users who want a refresher
Method development - how to get it right
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Method transfer - how to transfer your HPLC method
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
How to keep your HPLC healthy 24/7
Tips and tricks for keeping your HPLC healthy looking at the best practices. How to successfully troubleshoot issues with both your HPLC and methodology. What maintenance should be carried on your HPLC and when.
Learning Points:
- Keeping your instrument running
- Troubleshooting issues
- Maintenance of a HPLC
Who should attend:
- Users new to HPLC
- Users who want a refresher
HPLC troubleshooting - what is wrong with your HPLC?
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Performing maintenance on your HPLC systems
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Know your detectors – optical, charged aerosol detector, mass detector
An introduction to the principles of various HPLC detectors such as UV, fluorescence, charged aerosol detection. Also, a basic introduction to mass spectrometry for chromatographers.
Learning Points:
- Introductions to HPLC detectors
- Charged aerosol detectors
- Mass spectrometry introduction
Who should attend:
- Users new to HPLC
- Users who want a refresher
How do HPLC detectors work?
Mikael Glazier | Liquid/Gas Chromatography applications specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Charged Aerosol Detection - basic and beyond
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
Mass spectrometry for chromatographers - intro to MS
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Revealing the strength of HPLC-CAD methods
Imad Haidar Ahmad, PhD | Research Director, Amgen Wes Barnhart | Principal Scientist, Amgen
In recent years, the use of quantitative liquid chromatography (LC) coupled with charged aerosol detection (CAD) for poor UV absorbing analytes in multicomponent mixtures has grown exponentially across industrial sectors.
In this webinar, we will highlight some distinctions between CAD and other UV-absorbance detection methods, such as non-linearity in response to analyte concentration, and sensitivity to mobile phase impurities. Despite so, through careful optimization of CAD parameters, successful development, and transfer of analytical methods, nearly on par with that of conventional UV absorbance detectors, can be achieved. By meticulously tuning CAD parameters, we have developed quantitative LC-CAD assays which are sensitive, precise, accurate and robust across early and late-stage pharmaceutical development. Examples of LC-CAD methods employed at various stages of drug development, spanning from discovery to commercialization, will be shared during the presentation.
Learning points:
- Optimization: Optimizing non-linear detection response
- Troubleshooting: Tips and tricks to maintain a CAD detector
- Transfer: Examples of HPLC-CAD successfully developed and transferred (nearly on par with conventional HPLC-UV)
- Implementation of HPLC-MS-CAD for post µg-purification analysis
Who should attend:
- Analytical chemists: Professionals who specialize in analytical techniques, especially those involved in liquid chromatography and detection methods
- Pharmaceutical scientists: Researchers and scientists working in the pharmaceutical industry, particularly those involved in drug development and quality control
- Quality control and assurance professionals: Individuals responsible for ensuring the quality and reliability of analytical methods used in various industries, including pharmaceuticals
Imad Haidar Ahmad completed his graduate studies in Analytical Chemistry at Florida State University under Professor André Striegel. After graduation, he pursued postdoctoral training in separation science and analytical chemistry with Professor Peter W. Carr at the University of Minnesota. Imad began his professional career at Novartis in the Drug Inhalation department in San Carlos, CA, where he led method development efforts during various stages of drug development. He then joined Merck in Rahway, NJ, supervising the Method Screening & Purification group. Currently, Imad is a Director of Research at Amgen, focusing on method development, API purification, impurity isolation, and chromatography modeling.
Wesley W. Barnhart is a Principal Scientist on the Synthetic Separations team in Amgen's Research and Development department. He specializes in the analytical and preparative separations of achiral, chiral, and oligonucleotide molecules, as well as mass-directed purification of microgram quantities of pharmaceutical compounds.
Tips and tricks of HPLC
Dr. Susanne Fabel | HPLC Product Marketing Manager, Thermo Fisher Scientific
Mr. Sumit Verma | Regional Program Manager, Thermo Fisher Scientific
The webinar is for novice and experienced LC user, who wants to refresh their knowledge on the best practices of working with LC instrumentation.
Join Thermo Fisher Scientific experts on the flow path journey from sample injection to detection to learn useful tips on:
- Learn what might impact your chromatographic separation and detection, before you start your work.
- How to choose the right configuration and settings to achieve the best results.
- How to keep your system healthy and running.
By attending this webinar, you will learn to optimize your HPLC and UHPLC systems operations to achieve the best results for your LC separation.
Dr. Susanne Fabel is a Product Marketing Manager in the Chromatography and Mass Spectrometry group at Thermo Fisher Scientific. Her passion is to help develop world-class HPLC instruments to take science one step further. She has more than 15 years of experience in chromatography and holds a PhD in analytical chemistry from the Technical University of Munich.
Sumit Verma, is a distinguished Product Market Manager in the Asia-Pacific and Japan region. He is deeply associated with Chromatography and Mass spectrometry at Thermo Fisher Scientific, where he has amassed 18 years of rich experience in the Analytical Industry. Armed with a Post Graduation in Electronics and Management studies from IIMA India, Sumit embarked on his professional journey as a service engineer before venturing into Sales. Now, as a Product Marketing expert, he thrives on collaborating closely with customers to drive product improvement and innovation, aiming to simplify and enrich their lives.
Principles of HPLC
A basic introduction and overview of HPLC and the fundamental theories covering the following topics:
- What is HPLC?
- How a HPLC works
- Basic theory of separation
- Introduction to column chemistry
Learning Points:
- HPLC theory
- Basic column chemistry
- Hardware principles
Who should attend:
- Users new to HPLC
- Users who want a refresher
HPLC theory overview
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
A journey through a HPLC
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
Life isn’t all C18 - retaining tricky compounds
Looking at the alternatives to the standard approach of using C18 based phase. An opportunity more about some of the more unique column phases and how to optimize your chromatography.
Learning Points:
- Column chemistry
- Retaining problematic compounds
- Optimal conditions for unique phases
Who should attend:
- New to HPLC
- Users who want a refresher
Life beyond C18 - retaining tricky analytes
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Biopharma separation - large molecule analysis
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Method development using different HPLC column selectivities
Petra Gerhards | Regional Marketing Manager – Chromatography Consumables, Thermo Fisher Scientific
Petra´s background is over 30 years of experience in the field of GCMS, SPE and LCMS. Since joining the EMEA Regional Marketing Team for CCS she has been involved in a lot of workflow solutions combining Vials and Closures with SPE solutions, GCMS and LCMS. She works with KOL`s on data for regional specific marketing campaigns, organizes inhouse seminars and works on customer specific solutions. Her main expertise is in the field of Doping and Drugs of Abuse analysis.
Getting the most out of your HPLC – method development and transfer
The first part of the session will be focused on looking at method development covering the following topics:
- Using the right detector for your compounds
- Method optimization
- Choosing the right column
The second part of the session will be focused on method transfer covering the following topics:
- Transfer between instruments
- Method speed-up
- Important considerations to take on board when transferring methods
Learning Points:
- Method development
- Method transfer
- Introduction to UHPLC
Who should attend:
- Users new to HPLC
- Users who want a refresher
Method development - how to get it right
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Method transfer - how to transfer your HPLC method
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
How to keep your HPLC healthy 24/7
Tips and tricks for keeping your HPLC healthy looking at the best practices. How to successfully troubleshoot issues with both your HPLC and methodology. What maintenance should be carried on your HPLC and when.
Learning Points:
- Keeping your instrument running
- Troubleshooting issues
- Maintenance of a HPLC
Who should attend:
- Users new to HPLC
- Users who want a refresher
HPLC troubleshooting - what is wrong with your HPLC?
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Performing maintenance on your HPLC systems
Mikael Glazier | Liquid/Gas Chromatography Applications Specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Know your detectors – optical, charged aerosol detector, mass detector
An introduction to the principles of various HPLC detectors such as UV, fluorescence, charged aerosol detection. Also, a basic introduction to mass spectrometry for chromatographers.
Learning Points:
- Introductions to HPLC detectors
- Charged aerosol detectors
- Mass spectrometry introduction
Who should attend:
- Users new to HPLC
- Users who want a refresher
How do HPLC detectors work?
Mikael Glazier | Liquid/Gas Chromatography applications specialist, Thermo Fisher Scientific
Mikael Glazier is a chromatography specialist at Thermo Fisher Scientific, based in Staffordshire, UK. After finishing a degree in Chemistry, he joined British American Tobacco as a member of the method development and special projects team. During this time, he developed extensive knowledge of both LC-MS & GC-MS. After 10 years, Mikael left to manage a method development team in Harlan (a toxicology company focused on enabling customers to gain the required information for REACH), he then managed a small toxicology company responsible for multiple coroners within the UK. After that he joined Thermo Fisher Scientific in 2018 as a liquid chromatography & gas chromatography specialist, his role included working with customers in developing applications to help enable them to make the world healthier, cleaner and safer.
Charged Aerosol Detection - basic and beyond
Chris Tuczemskyi | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Chris Tuczemskyi is a chromatography specialist at Thermo Fisher Scientific, based in Cambridge, UK. After spending a number of years building up his experience with chromatography instruments in a water testing laboratory, he joined Thermo Fisher Scientific in 2014 as a chromatography service engineer for Unity Lab Services. Here he developed his passion for supporting customers using HPLC and in 2018 moved into the application specialist team. As a chromatography specialist, his role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Chris graduated from the University of Essex in Biochemistry (Bsc Hons).
Mass spectrometry for chromatographers - intro to MS
Alexandra Graham | Liquid Chromatography Applications Specialist, Thermo Fisher Scientific
Alex Graham is a chromatography specialist at Thermo Fisher Scientific, based in Hertfordshire, UK. After finishing a Masters degree in Toxicolology, she joined HFL Sport Science using a variety of chromatography instruments and gaining experience in anti-doping testing and toxicology. After 9 years in that industry, she joined Thermo Fisher Scientific in 2018 as a liquid chromatography specialist, her role includes working with customers in developing applications to help to enable them to make the world healthier, cleaner and safer. In 2009, Alex graduated from Keele University with a degree in Medicinal chemistry and Forensic science (Bsc Hons) and then graduated in 2010 from Kings College London with Masters in Analytical toxicology.
Revealing the strength of HPLC-CAD methods
Imad Haidar Ahmad, PhD | Research Director, Amgen Wes Barnhart | Principal Scientist, Amgen
In recent years, the use of quantitative liquid chromatography (LC) coupled with charged aerosol detection (CAD) for poor UV absorbing analytes in multicomponent mixtures has grown exponentially across industrial sectors.
In this webinar, we will highlight some distinctions between CAD and other UV-absorbance detection methods, such as non-linearity in response to analyte concentration, and sensitivity to mobile phase impurities. Despite so, through careful optimization of CAD parameters, successful development, and transfer of analytical methods, nearly on par with that of conventional UV absorbance detectors, can be achieved. By meticulously tuning CAD parameters, we have developed quantitative LC-CAD assays which are sensitive, precise, accurate and robust across early and late-stage pharmaceutical development. Examples of LC-CAD methods employed at various stages of drug development, spanning from discovery to commercialization, will be shared during the presentation.
Learning points:
- Optimization: Optimizing non-linear detection response
- Troubleshooting: Tips and tricks to maintain a CAD detector
- Transfer: Examples of HPLC-CAD successfully developed and transferred (nearly on par with conventional HPLC-UV)
- Implementation of HPLC-MS-CAD for post µg-purification analysis
Who should attend:
- Analytical chemists: Professionals who specialize in analytical techniques, especially those involved in liquid chromatography and detection methods
- Pharmaceutical scientists: Researchers and scientists working in the pharmaceutical industry, particularly those involved in drug development and quality control
- Quality control and assurance professionals: Individuals responsible for ensuring the quality and reliability of analytical methods used in various industries, including pharmaceuticals
Imad Haidar Ahmad completed his graduate studies in Analytical Chemistry at Florida State University under Professor André Striegel. After graduation, he pursued postdoctoral training in separation science and analytical chemistry with Professor Peter W. Carr at the University of Minnesota. Imad began his professional career at Novartis in the Drug Inhalation department in San Carlos, CA, where he led method development efforts during various stages of drug development. He then joined Merck in Rahway, NJ, supervising the Method Screening & Purification group. Currently, Imad is a Director of Research at Amgen, focusing on method development, API purification, impurity isolation, and chromatography modeling.
Wesley W. Barnhart is a Principal Scientist on the Synthetic Separations team in Amgen's Research and Development department. He specializes in the analytical and preparative separations of achiral, chiral, and oligonucleotide molecules, as well as mass-directed purification of microgram quantities of pharmaceutical compounds.
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