Gibco Feeds and Supplements

Cell culture operations typically use three categories of formulations—nutrient feeds, metabolic supplements, and process additives. Nutrient feeds are formulated to supply concentrated components, such as amino acids, vitamins, glucose, and trace elements, to help offset depletion during extended culture. Metabolic supplements can adjust specific elements that influence cell growth or product formation. Additives are used to help address defined needs, such as pH balance, osmolality, or product quality attributes. Each category is applied based on cell line behavior and the demands of the culture format.

In bioprocessing, feeds and Gibco supplements are designed to help manage changes that occur as cultures increase in density and nutrient consumption rises. In fed-batch systems, defined feeding schedules replace depleted components and extend the productive phase of the culture. In perfusion systems, supplements help maintain stable conditions as the medium is exchanged continuously. These adjustments allow processes to maintain viability, manage metabolic shifts, and produce consistent material across development and scale-up.

Gibco feeds and supplements are used in CHO-based monoclonal antibody (mAb) production to help manage nutrient availability, sustain viability, and maintain consistent expression levels during fed-batch culture. EfficientFeed Supplements allow concentrated components to meet the increased metabolic demands of high-density cultures, while FunctionMAX TiterEnhancer is used to help improve protein expression without altering established process conditions. GlycanTune Total Feeds can be applied when specific glycan patterns are required for mAb product quality. These formulations integrate into standard upstream workflows used across development and manufacturing.
 

Discover mAb bioprocessing solutions to enable efficient scale-up from clinical to commercial manufacturing.

Feeds and supplements can improve yield by replacing nutrients that are consumed as cultures reach higher densities. Sustained availability of amino acids, trace elements, and carbon sources helps maintain viable cell populations and steady protein expression. Supplements can also adjust specific components that influence growth rate, productivity, or product characteristics. Together, these additions help stabilize culture behavior over longer runs and contribute to more consistent process performance.

Gibco feeds and supplements are manufactured under cGMP conditions with documented raw materials, traceability, and defined quality controls. Each lot is released with the documentation needed for qualification, and animal origin–free (AOF) options are available when required. These materials are used across development and manufacturing settings where performance and regulatory alignment are important.

Advanced feed formulations allow higher nutrient delivery without increasing working volume, which helps manage the constraints that arise as bioreactors run at higher cell densities. Their concentrated formats address solubility limits, reduce dilution effects, and enable controlled feeding strategies during longer operations. These characteristics help maintain stable conditions within stirred-tank and single-use bioreactors as processes move from development to production.