Verified antibody panels for flow cytometry

Verified antibody panels for flow cytometry

Coined in 2010, the term “optimized multicolor immunofluorescence panel”, or OMIP, refers to a thoroughly tested and validated set of antibodies and reagents that can be used together for the multicolor characterization of a specific cell state or response. Published in the journal Cytometry Part A (Wiley Online Library), these OMIPs are designed for flow cytometry, but OMIPs may potentially be defined for image cytometry, fluorescence microscopy, and other polychromatic fluorescence–based methods.


Articles in Cytometry Part A about OMIPs are listed in the table below.

Locate the OMIP you’re interested in, using the table’s search and sort functions. Clicking on the linked text will take you to the article.

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OMIP IDOMIP nameTarget cell type
OMIP-001Quality and phenotype of Ag-responsive human T cells  Human T cells
OMIP-002Phenotypic analysis of specific human CD8+ T-cells using peptide-MHC class I multimers for any of four epitopes  Human T cells
OMIP-003Phenotypic analysis of human memory B cells  Human B cells
OMIP-004In-depth characterization of human T regulatory cells  Human T cells
OMIP-005Quality and phenotype of antigen-responsive rhesus macaque T cells  Rhesus macaque T cells
OMIP-006Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry  Human T cells
OMIP-007A phenotypic analysis of human natural killer cells  Human NK cells
OMIP-008Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells  Human T cells
OMIP-009Characterization of antigen-specific human T cells  Human T cells
OMIP-010A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples  Human hematopoietic cells
OMIP-011Characterization of circulating endothelial cells (CECs) in peripheral blood  Human endothelial cells
OMIP-012Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice  Human leukocytes in humanized mouse
OMIP-013Differentiation of human T-cells  Human T cells
OMIP-014Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining  Human T cells
OMIP-015Human regulatory and activated T-cells without intracellular staining  Human T cells
OMIP-016Characterization of antigen-responsive macaque and human T-cells  Cynomolgus macaque or Human T Cells
OMIP-017Human CD4+ helper T-cell subsets including follicular helper cells  Human T cells
OMIP-018Chemokine receptor expression on human T helper cells  Human T cells
OMIP-019Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors Human T cells, NK cells
OMIP-020Phenotypic characterization of human γδ T-cells by multicolor flow cytometry  Human T cells
OMIP-021Simultaneous quantification of human conventional and innate-like T-cell subsets  Human T cells
OMIP-022Comprehensive assessment of antigen-specific human T-cell functionality and memory  Human T cells
OMIP-02310-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes  Human leukocytes
OMIP-024Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samplesHuman leukocytes
OMIP-025Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining  Human T cells, NK cells
OMIP-026Phenotypic analysis of B and plasma cells in rhesus macaques  Rhesus macaque B cells, plasma cells
OMIP-027Functional analysis of human natural killer cells  Human NK cells
OMIP-028Activation panel for rhesus macaque NK cell subsets  Rhesus macaque NK cells
OMIP-029Human NK-cell phenotypization  Human NK cells
OMIP-030Characterization of human T cell subsets via surface markers  Human T cells
OMIP-031Immunologic checkpoint expression on murine effector and memory T-cell subsets  Mouse T cells
OMIP-032Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland  Mouse leukocytes
OMIP-033A comprehensive single step staining protocol for human T- and B-cell subsets  Human T cells, B cells
OMIP-034Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies  Human leukocytes
OMIP-035Functional analysis of natural killer cell subsets in macaques  Rhesus macaque NK cells
OMIP-036Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets  Human T cells
OMIP-03716-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets  Human lymphocytes
OMIP-038Innate immune assessment with a 14 color flow cytometry panelHuman lymphocytes
OMIP-039Detection and analysis of human adaptive NKG2C+ natural killer cellsNK cells, lymphocytes
OMIP-040Optimized gating of human prostate cellular subpopulationsHuman leukocytes, epithelia, and stroma
OMIP-041Optimized multicolor immunofluorescence panel rat microglial staining protocolRat primary microglia cells
OMIP-04221‐color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral bloodHuman PBMCs
OMIP-043Identification of human antibody secreting cell subsetsHuman Fresh or cryopreserved PBMC, bone marrow, spleen, or tonsil cells
OMIP-04428-color immunophenotyping of the human dendritic cell compartmentHuman dendritic cells
OMIP-045Characterizing human head and neck tumors and cancer cell lines with mass cytometryCancer cell lines
OMIP-046Characterization of invariant T cell subset activation in humansHuman PBMCs
OMIP-047High‐Dimensional phenotypic characterization of B cellsHuman PBMCs
OMIP-048 MCQuantification of calcium sensors and channels expression in lymphocyte subsets by mass cytometryPrimary murine lymphocytes from spleen and lymph nodes
OMIP-049Analysis of Human Myelopoiesis and Myeloid NeoplasmsHuman myeloid cells
OMIP-050A 28‐color/30‐parameter Fluorescence Flow Cytometry Panel to Enumerate and Characterize Cells Expressing a Wide Array of Immune Checkpoint MoleculesHuman PBMCs
OMIP-05128-color flow cytometry panel to characterize B cells and myeloid cellsHuman B cells
OMIP-052An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus MacaquesRhesus macaque PBMCs
OMIP-053Identification, Classification, and Isolation of Major FoxP3 Expressing Human CD4+ Treg SubsetsHuman PBMCs
OMIP-054Broad Immune Phenotyping of Innate and Adaptive Leukocytes in the Brain, Spleen, and Bone Marrow of an Orthotopic Murine Glioblastoma Model by Mass CytometryMouse T lymphocytes
OMIP-055Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral BloodHuman lymphoid cells
OMIP-056Evaluation of Human Conventional T Cells, Donor‐Unrestricted T Cells, and NK Cells Including Memory Phenotype by Intracellular Cytokine StainingCryopreserved PBMCs
OMIP-057Mouse γδ T‐Cell Development Characterized by a 14 Color Flow Cytometry PanelMouse thymocytes
OMIP-05830‐Parameter Flow Cytometry Panel to Characterize iNKT, NK, Unconventional and Conventional T CellsHuman PBMCs
OMIP-059Identification of Mouse Hematopoietic Stem and Progenitor Cells with Simultaneous Detection of CD45.1/2 and Controllable Green Fluorescent Protein Expression by a Single Staining PanelMouse hematopoietic Stem cells
OMIP-06030‐Parameter Flow Cytometry Panel to Assess T Cell Effector Functions and Regulatory T CellsHuman PMBCs
OMIP-06120‐Color Flow Cytometry Panel for High‐Dimensional Characterization of Murine Antigen‐Presenting CellsMouse dendritic cells
OMIP-062A 14‐Color, 16‐Antibody Panel for Immunophenotyping Human Innate Lymphoid, Myeloid and T Cells in Small Volumes of Whole Blood and Pediatric Airway SamplesHuman PBMCs
OMIP-06328‐Color Flow Cytometry Panel for Broad Human ImmunophenotypingHuman PBMCs
OMIP-064A 27‐Color Flow Cytometry Panel to Detect and Characterize Human NK Cells and Other Innate Lymphoid Cell Subsets, MAIT Cells, and γδ T CellsHuman PBMCs
OMIP-065Dog Immunophenotyping and T‐Cell Activity Evaluation with a 14‐Color PanelDog PBMCs
OMIP-066Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral BloodHuman PBMCs
OMIP-06728‐Color Flow Cytometry Panel to Evaluate Human T‐Cell Phenotype and FunctionHuman PBMCs
OMIP-068High‐Dimensional Characterization of Global and Antigen‐Specific B Cells in Chronic InfectionHuman PBMCs
OMIP-069Forty-Color Full Spectrum Flow CytometryPanel for Deep Immunophenotyping of Major CellSubsets in Human Peripheral BloodHuman leukocytes
OMIP-070NKp46‐Based 27‐Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor TissuesHuman tumor cells
OMIP-071A 31‐Parameter Flow Cytometry Panel for In‐Depth Immunophenotyping of Human T‐Cell Subsets Using Surface MarkersHuman T-Cells
OMIP-072A 15‐color panel for immunophenotypic identification, quantification, and characterization of leukemic stem cells in children with acute myeloid leukemiaLeukemic stem cells
OMIP-073Analysis of human thymocyte development with a 14‐color flow cytometry panelHuman Thymocytes
OMIP-074Phenotypic analysis of IgG and IgA subclasses on human B cellsHuman PBMCs
OMIP-075A 22-color panel for the measurement of antigen-specific T-cell responses in human and nonhuman primatesHuman and nonhuman primate PBMCs
OMIP-076High-dimensional immunophenotyping of murine T-cell, B-cell, and antibody secreting cell subsetsMouse T-cells
OMIP-077Definition of all principal human leukocyte populations using a broadly applicable 14-color panelHuman leukocytes
OMIP-078A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cellsHuman T-cells
OMIP-079Cell cycle of CD4+ and CD8+ naïve/memory T cell subsets, and of Treg cells from mouse spleenMouse splenocytes
OMIP-08029-Color flow cytometry panel for comprehensive evaluation of NK and T cells reconstitution after hematopoietic stem cells transplantationHuman PBMCs
OMIP-081A new 21-monoclonal antibody 10-color panel for diagnostic polychromatic immunophenotypingHuman bone marrow, peripheral blood
OMIP-082A 25-color phenotyping to define human innate lymphoid cells, natural killer cells, mucosal-associated invariant T cells, and γδ T cells from freshly isolated human intestinal tissueHuman innate lymphoid cells, NK cells, MAIT cells, gamma/delta T cells
OMIP-083A 21-marker 18-color flow cytometry panel for in-depth phenotyping of human peripheral monocytesHuman monocytes
OMIP-08428-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cellsHuman T cells, gamma/delta T cells, MAIT cells
OMIP-085Cattle B-cell phenotyping by an 8-color panelCattle B cells
OMIP-086Full spectrum flow cytometry for high-dimensional immunophenotyping of mouse innate lymphoid cellsMouse innate lymphoid cells, small intestine lamina propria
OMIP-087Thirty-two parameter mass cytometry panel to assess human CD4 and CD8 T cell activation, memory subsets, and helper subsetsHuman CD4 and CD8 T cells
OMIP-088Twenty-target imaging mass cytometry panel for major cell populations in mouse formalin fixed paraffin embedded liverMouse liver FFPE
OMIP-089Cattle T-cell phenotyping by an 8-color panelCattle T cells
OMIP-090A 20-parameter flow cytometry panel for rapid analysis of cell diversity and homing capacity in human conventional and regulatory T cellsHuman T regulatory (Treg) cells
OMIP-091A 27-color flow cytometry panel to evaluate the phenotype and function of human conventional and unconventional T-cellsHuman T cells, gamma/delta T cells, MAIT cells, Memory T cells, Th1, Th2, Th17, Tfh, Treg
OMIP-092Characterization of rat macrophage subsets, lymphocytes, and granulocytes in bronchoalveolar lavage fluidRat macrophages, lymphocytes, granulocytes
OMIP-093A 41-color high parameter panel to characterize various co-inhibitory molecules and their ligands in the lymphoid and myeloid compartment in miceMouse immunophenotyping
OMIP-094Twenty-four-color (thirty-marker) panel for deep immunophenotyping of immune cells in human peripheral bloodHuman immunophenotyping
OMIP-09540-Color spectral flow cytometry delineates all major leukocyte populations in murine lymphoid tissuesMouse immunophenotyping
OMIP-096A 24-color flow cytometry panel to identify and characterize CD4+ and CD8+ tissue-resident T cells in human skin, intestinal, and type II mucosal tissueHuman CD4 and CD8 T cells
OMIP-097High-parameter phenotyping of human platelets by spectral flow cytometryHuman platelets
OMIP-098A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotypingNK cell phenotype, using cryopreserved PBMCs
OMIP-09931-color spectral flow cytometry panel to investigate the steady-state phenotype of human T cellsSteady-state phenotype of human T cell
OMIP-100A flow cytometry panel to investigate human neutrophil subsetsNeutrophils
OMIP-10127-color flow cytometry panel for immunophenotyping of major leukocyte populations in fixed whole bloodLeukocyte subsets - myeloid and lymphoid cell populations
OMIP-10250-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cellsPBMCs and human tissue samples
OMIP-103A 35-marker imaging mass cytometry panel for the co-detection of HIV and immune cell populations in human formalin fixed paraffin embedded intestinal tissueImmune cell populations and HIV RNA in FFPE-human intestinal tissue
OMIP-104A 30-color spectral flow cytometry panel for comprehensive analysis of immune cell composition and macrophage subsets in mouse metabolic organsMurine metabolic tissues and metabolically-relevant myeloid cell subsets
OMIP-105A 30-color full-spectrum flow cytometry panel to characterize the immune cell landscape in spleen and tumor within a syngeneic MC-38 murine colon carcinoma modelCD4 T cells, CD8 T cells, regulatory T cells, NK cells, B cells, macrophages, granulocytes, monocytes, and dendritic cells
OMIP-106A 30-color panel for analysis of check-point inhibitory networks in the bone marrow of acute myeloid leukemia patientsEffector immune cell (NK cells, γδ T cells, NKT-like T cells, and classical T cells; hematopoietic stem and progenitor cells)
OMIP-1078-color whole blood immunophenotyping panel for the characterization and quantification of lymphocyte subsets and monocytes in swineMajor lymphocyte subsets and monocytes in pigs
OMIP-10822-color flow cytometry panel for detection and monitoring of chimerism and immune reconstitution in porcine-to-baboon models of operational xenotransplant tolerance studiesPorcine myeloid and lymphoid lineages; major lymphoid lineages in baboons
OMIP-10945-color full spectrum flow cytometry panel for deep immunophenotyping of the major lineages present in human peripheral blood mononuclear cells with emphasis on the T cell memory compartmentHuman T cells, γδ T cells, NKT-like cells, B cells, NK cells, monocytes, basophils, dendritic cells, and ILCs
OMIP-110A 37-Color Spectral Flow Cytometric Panel to Assess Transcription Factors and Chemokine Receptors in Human Intestinal Lymphoid CellsHuman ILC, MAIT, NK and T cells in PBMCs
OMIP-111Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular CytokinesT cells, memory phenotypes, and functional cytokines in murine splenocytes
OMIP-11242-Parameter (40-Color) Spectral Flow Cytometry Panel for Comprehensive Immunophenotyping of Human Peripheral Blood LeukocytesNeutrophils, basophils, eosinophils, monocytes, dendritic cells, T cells, MAIT cells, γδ T cells, B cells, NK cells, DCs, and ILCs
OMIP-113Characterization of Cytokine Producing T Cells in Swineαβ and γδ T cells from PBMCs
OMIP-114A 36-Color Spectral Flow Cytometry Panel for Detailed Analysis of T Cell Activation and Regulation in Small Human Blood VolumesSubpopulations within T cells, B cells and NK cells from PBMCs
OMIP-115High-Dimensional Phenotypic Characterization of Human Natural Killer Cells for Therapeutic UseHuman peripheral blood NK cells