GeneArt HTP Antibody Expression Service

Efficient, high-throughput antibody production is crucial for research and development. Our GeneArt high-throughput (HTP) antibody expression service is designed to meet the growing demand for high-throughput antibody production, providing consistent and scalable solutions for your research needs.

Reliable small-scale HTP antibody expression services

When your research focuses on discovery and early development of a therapeutic antibody, having reliable, high-quality proteins from the start can reduce the risk of late-stage failures. Our semi-automated, high-throughput platform allows for reproducible and rapid production of antibodies from transiently transfected mammalian cells, while remaining flexible enough to address the needs of the rapidly evolving immunotherapy field.

As with our mid- and large-scale protein expression services, high yields are achieved with a combination of outstanding sequence optimization and high-efficiency Gibco ExpiCHO, Expi293, or Expi293 PRO expression systems.

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HTP antibody expression and recovery consistency

To prevent false negatives due to poor yield, it is important to utilize a platform that maximizes expression consistently within and between production runs. Figure 1 demonstrates reproducible expression and recovery of antibodies across independent runs.

Scatter plot illustrating reproducibility of antibody expression and recovery for 38 antibodies in ExpiCHO (left) and Expi293 cells (right), using protein A/G magnetic beads

Figure 1. Reproducible antibody expression and recovery. Protein concentration for 38 antibodies, expressed in ExpiCHO (left) or Expi293 cells (right) and purified with protein A/G magnetic beads, correlate well between independent production runs.

To further prevent candidate dropout later in development, expression levels should be consistent as you scale up to larger cultures. Figure 2 demonstrates consistent relative expression between small-scale, high-throughput cultures (2.5 mL) and either 200 mL or 1 L cultures.

Scatter plot illustrating protein titers from supernatant in ExpiCHO cells (10 days, left) and Expi293 cells (7 days, right)

Figure 2. Consistent relative expression between small-scale and high-throughput culture volumes. Protein titer from supernatant after 10 days expression in ExpiCHO (left) or 7 days expression in Expi293 cells (right) correlate well between small scale HTP (2.5 mL) and 200 mL or 1 L cultures.

High-throughput antibody production service details

Deliverables	Antibody in Matrix 2D barcoded tubes (default) 5 µg of plasmid DNA COA/Data sheet
Expression systems	ExpiCHO Expi293 Expi293 PRO
Estimated delivered yield	ExpiCHO or Expi293: 0.1–1 mg Expi293 PRO: Up to 3 mg
Purification options	Supernatant (no purification) One-step protein A/G purification
QC analytics	Purified antibodies Purity assessment (CE-SDS) Total protein quantitation using UV/Vis-DLS Stunner Volume determination by ultrasonic distance measurement Supernatant Octet BLI titer measurement Volume determination by ultrasonic distance measurement
Production time	15–20 business days including gene synthesis
Optional add-ons	Alternative delivery in 96-well deep well plate or FluidX tubes Endotoxin measurement In-process Octet BLI titer measurement for purified antibodies

Easy ordering for small-scale HTP antibodies

Whether you know exactly what you need or you would like a little advice, it helps to have a technical team available to guide you through the process from quote to project delivery. Our project management team is ready to provide the level of support you need.

If you have your sequences and are ready to order, use our online quote request tool to help you provide all the information we need to prepare a prompt quote for your project.

If you still have questions, please reach out to the team directly at geneartpmo@thermofisher.com.