Plasmid DNA (pDNA) aids in transfer or expression of genes which are fundamental to biological research and therapeutic development. When used to directly express genes, it is necessary to scale up production of high-quality, transfection-ready pDNA.

Use GeneArt Plasmid Purification Services to get transfection-ready custom plasmid DNA that meet regulatory requirements. Our services encompass both pDNA preparation and purification. Our offerings include both research-grade pDNA and pre-clinical grade pDNA. We produce pDNA at a range of scales from 50 mL to 5 L (Table 1) with add-on services such as restriction analysis, endotoxin testing, and bioburden testing. Our low-endotoxin pDNA preparations are suitable for early development and pre-clinical research.

All purified plasmids undergo rigorous quality control and include quality assurance documentation. Our GeneArt facilities are ISO9001:2015 certified and use proven protocols, quality-controlled systems, and reagents.


Ordering plasmid purification services

The GeneArt Services Dashboard offers the easiest way to order

  • Build your own construct with gene synthesis and add plasmid purification
  • Access all your projects and monitor order status
  • Re-order plasmid purification for an archived construct

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Reasons to choose GeneArt plasmid services

equipment utilized in GeneArt Plasmid Purification Services

The GeneArt Plasmid Purification process produces highly pure, homogenous pDNA ready for transfection and transformation. GeneArt Plasmid Services offers the following:

  • Extremely low levels of endotoxin: <0.1 EU/µg pDNA in selected scales (generally considered endotoxin-free)
  • Scaleup: microgram to milligram scale production
  • Completed in as few as 3 business days following gene synthesis
  • Delivery in liquid or frozen on dry ice
  • Custom aliquoting and tube labeling
Scales & yields

Plasmid preparation services: Scales & yields

Table 1. Options for plasmid DNA purification scales with yields.
Plasmid purification scaleTypical yieldsConcentrationRestriction analysisEndotoxin testBioburden testing
Midiprep (50 mL)

~100 µg pDNA
(high performing plasmids)

~10 µg pDNA
(low performing plasmids)

Standard:
1 mg/mL

On request:
0.1, 0.5, 2.0, 4.0 mg/mL		NANANA
Maxiprep* (250 mL)

~500 µg pDNA
(high performing plasmids)

~50 µg pDNA
(low performing plasmids)

NANANA
Megaprep*
1 mg guaranteed

~1 mg pDNA
(high performing plasmids)

~100 µg pDNA
(low performing plasmids)

OptionalOptionalNA
Gigaprep* (2.5 L)

~5 mg pDNA
(high performing plasmids)

~1 mg DNA
(low performing plasmids)

OptionalYesOptional
2x Gigaprep* (5 L)

~10 mg pDNA
(high performing plasmids)

~2 mg DNA
(low performing plasmids)

OptionalYesOptional

* Prepared with low endotoxin plasmid prep technology.

Documentation & QC

Plasmid purification services: Documentation & quality control

Plasmid DNA purification specifications and quality control include:

  • Appearance by visual inspection
  • Purity via A260/A280
  • Closed circular construct >85% (visual inspection)
  • Transmissible spongiform encephalopathy (TSE)-free option available
  • Sequence verification of gene of interest
  • Restriction analysis available for larger scales
  • Low endotoxin (larger scales): <100 EU/mL
  • Buffer options: TE, H2O, PBS

Applicable quality control data, based on selected scale and options, provided on the standard Certificate of Analysis (CofA).

Additional documentation available upon request:

  • Material documentation—Documentation of gene construction including gene design, cloning strategy, oligo design, chemical and enzymes (provider and Cat. No.), releasing scientist, shipping provider, and tracking number. Additional documentation can include a working / SOP list and documentation of releasing scientist.
  • Data storage for material documentation—Storage of all relevant data for material documentation for 10 years. Material documentation report can be requested at any time during these 10 years.


Related products and services

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Contact us

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North America and Asia: 1-800-955-6288
Europe: +49 (0)941 942 76-100

Order support:
Geneartsupport@thermofisher.com

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