Technical Resources - Media Formulations

42360 - MEM, HEPES, GlutaMAX(TM)

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
L-Alanyl-Glutamine217.0434.02.0
L-Arginine hydrochloride211.0126.00.5971564
L-Cystine 2HCl313.031.00.09904154
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.052.00.39694658
L-Leucine131.052.00.39694658
L-Lysine hydrochloride183.073.00.3989071
L-Methionine149.015.00.10067114
L-Phenylalanine165.032.00.19393939
L-Threonine119.048.00.40336135
L-Tryptophan204.010.00.04901961
L-Tyrosine disodium salt dihydrate261.052.00.19923371
L-Valine117.046.00.3931624
Vitamins
Choline chloride140.01.00.007142857
D-Calcium pantothenate477.01.00.002096436
Folic Acid441.01.00.0022675737
Niacinamide122.01.00.008196721
Pyridoxal hydrochloride204.01.00.004901961
Riboflavin376.00.12.6595744E-4
Thiamine hydrochloride337.01.00.002967359
i-Inositol180.02.00.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Magnesium Sulfate (MgSO4) (anhyd.)142.097.670.6878169
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.02200.026.190475
Sodium Chloride (NaCl)58.06350.0109.48276
Sodium Phosphate monobasic (NaH2PO4-H2O)140.0140.01.0
Other Components
D-Glucose (Dextrose)180.01000.05.5555553
HEPES238.05958.025.033613
Phenol Red376.410.00.026567481

Referencia

1. Biol. Med. Sci., 61:439. (rat astrocytes).

2. Eagle, H. (1959) Science 130:432.

3. Lolait, S.J., Harmer, J.H., Dahl, D., et al., (1983) Aust. J. Exp.

4. Norris, S.J. and Edmondson, D.G. (1986) Sex. Transm. Dis., 13:207 (Rabbit epithelial cells).

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