Technical Resources - Media Formulations

Neurobasal™-A Medium (1X) liquid

Contains no glucose or sodium pyruvate.

Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements. They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer. The concentration of NaCl is adjusted to bring the Osmolality to 260 +/- 10 mOsm

  • Catalog Number(s)

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.030.00.4
L-Alanine89.02.00.02247191
L-Arginine hydrochloride211.084.00.39810428
L-Asparagine-H2O150.00.830.0055333334
L-Cysteine121.031.50.2603306
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.0105.00.8015267
L-Leucine131.0105.00.8015267
L-Lysine hydrochloride183.0146.00.7978142
L-Methionine149.030.00.20134228
L-Phenylalanine165.066.00.4
L-Proline115.07.760.06747826
L-Serine105.042.00.4
L-Threonine119.095.00.79831934
L-Tryptophan204.016.00.078431375
L-Tyrosine181.072.00.39779004
L-Valine117.094.00.8034188
Vitamins
Choline chloride140.04.00.028571429
D-Calcium pantothenate477.04.00.008385744
Folic Acid441.04.00.009070295
Niacinamide122.04.00.032786883
Pyridoxal hydrochloride204.04.00.019607844
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.04.00.011869436
Vitamin B121355.00.00685.0184503E-6
i-Inositol180.07.20.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Ferric Nitrate (Fe(NO3)3"9H2O)404.00.12.4752476E-4
Magnesium Chloride (anhydrous)95.077.30.8136842
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.02200.026.190475
Sodium Chloride (NaCl)58.04000.068.965515
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0125.00.9057971
Zinc sulfate (ZnSO4-7H2O)288.00.1946.736111E-4
Other Components
HEPES238.02600.010.92437
Phenol Red110.08.10.07363637

Referencia