Generational tracing using CellTrace™ CFSE

Cell proliferation was followed for 7 generations using the CellTrace™ CFSE reagent. Human T lymphocytes were harvested and stained with CellTrace™ CFSE prior to stimulation with anti-human CD3 (Cat. no. MHCD0300) for 5 days. The discrete peaks in these histograms represent successive generations of live, SYTOX® Green (Cat. no. S34860) negative cells. The unstimulated parent generation is indicated in blue. Analysis was completed using an Attune® Acoustic Focusing Cytometer with 488 nm excitation and a 530/30 nm bandpass emission filter.

Cell proliferation was followed for 7 generations using the CellTrace™ CFSE reagent. Human T lymphocytes were harvested and stained with CellTrace™ CFSE prior to stimulation with anti-human CD3 (Cat. no. MHCD0300) for 5 days. The discrete peaks in these histograms represent successive generations of live, SYTOX® Green (Cat. no. S34860) negative cells. The unstimulated parent generation is indicated in blue. Analysis was completed using an Attune® Acoustic Focusing Cytometer with 488 nm excitation and a 530/30 nm bandpass emission filter.

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