In this webinar, strategies for improving PTM detection and quantitation in scarce clinical samples, and clinical research applications of SRM-based assays will be presented.
Analysis of histone modifications using existing mass spectrometry (MS)-based methods requires large numbers of cells, however for some clinical specimens, it is extremely difficult to obtain a large number of cells. An action plan was outlined to establish and validate a full pipeline to quantitate all major and rare histone modifications from 106 down to 103 cells. To accomplish this feat, a two-pronged approach was proposed using two different MS strategies: full-length histone analysis (top-down) and triple-quadrupole based tryptic peptide quantitation (bottom-up). In this webinar, current and future technology improvements that will significantly improve the limits of detection and quantitation of existing SRM-based bottom-up approach are presented.
