The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Invitrogen Alexa Fluor 546 dye is a bright, yellow-fluorescent dye that can be excited using the 488 nm or 532 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-insensitive over a wide molar range.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor 546 dye dashboard
 Initial brightness |
An alternative for tetramethylrhodamine or Cy3 dye, with higher initial intensity than Alexa Fluor 555 dye, but lower photostability in buffer or antifade. | ||
 Photostability in buffer |
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 Photostability in antifade |
| 488/532 | TRITC | 556 | 573 | ||
 Laser line |
 Common filter set |
 Excitation max |
 Emission max |
Alexa Fluor 546 products
We offer a broad range of Alexa Fluor 546 products including labeling kits, secondary antibodies, bioconjugates, and more.
Learn more here:
- Antibody labeling kits for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Hydrazides for neuronal tracing
- Labeled nucleotides for hybridization probes
The intermediate filaments in bovine pulmonary artery endothelial cells were visualized using our anti-desmin antibody, followed by Alexa Fluor 647 goat anti–mouse IgG antibody (purple). Endogenous biotin in the mitochondria was labeled with Alexa Fluor 546 streptavidin (red), and DNA in the cell was stained with DAPI (white).
Alexa Fluor 546 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 546 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Alexa Fluor 546 Mouse IgG1 Labeling Kit | Z25004 |
| <1–20 µg rabbit IgG | Zenon Alexa Fluor 546 Rabbit IgG Labeling Kit | Z25304 |
| Conventional antibody/protein labeling | ||
| 100 µg antibody/protein | Alexa Fluor 546 Antibody/Protein Labeling Kit | A20183 |
| 1 mg antibody/protein | Alexa Fluor 546 Antibody/Protein Labeling Kit | A10237 |
Alexa Fluor dye family
Illuminating research for over 15 years
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
Resources
Molecular Probes Handbook
Antifade reagents
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
See the performance of this dye in SlowFade Antifade reagents
See the performance of this dye in ProLong Antifade reagents
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.