Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Invitrogen Alexa Fluor 555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
We offer Alexa Fluor 555 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor 555 dye dashboard
 Initial brightness |
Recommended replacement for tetramethylrhodamine or Invitrogen Cy3 dye, exhibiting greater photostability than Cy3 dye in live and fixed cells. In addition, is used in stochastic optical reconstruction microscopy (STORM)—as an activator in nSTORM and a reporter in dSTORM. | ||
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 Photostability in antifade |
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 Performance in SRM |
| 488/532 | TRITC | 555 | 580 | ||
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Alexa Fluor 555 products
We offer a broad range of Alexa Fluor 555 products including labeling kits, secondary antibodies, bioconjugates, and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits
- Primary antibodies for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Annexin V conjugates for apoptosis imaging
- Hydrazides for neuronal tracing
- Labeled nucleotides for hybridization probes
A muntjac skin fibroblast was fixed and permeabilized. Mitochondria were labeled with anti–Oxphos Complex V inhibitor protein mouse IgG1 and visualized using orange-fluorescent Invitrogen Alexa Fluor 555 goat anti–mouse IgG. F-actin was labeled with green-fluorescent Invitrogen Alexa Fluor 488 phalloidin, and the nucleus was stained with Invitrogen TO-PRO 3 iodide (pseudocolored magenta).
Alexa Fluor 555 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 555 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Alexa Fluor 555 Mouse IgG1 Labeling Kit | Z25005 |
| <1–20 µg mouse IgG2a | Zenon Alexa Fluor 555 Mouse IgG2a Labeling Kit | Z25105 |
| <1–20 µg mouse IgG2b | Zenon Alexa Fluor 555 Mouse IgG2b Labeling Kit | Z25205 |
| <1–20 µg rabbit IgG | Zenon Alexa Fluor 555 Rabbit IgG Labeling Kit | Z25305 |
| Conventional antibody/protein labeling | ||
| 10–20 µg antibody | APEX Alexa Fluor 555 Antibody Labeling Kit | A10470 |
| 20–100 µg antibody/protein | Alexa Fluor 555 Microscale Antibody/Protein Labeling Kit | A30007 |
| 100 µg antibody/protein | Alexa Fluor 555 Antibody/Protein Labeling Kit | A20187 |
| 1 mg antibody/protein | Alexa Fluor 555 Antibody/Protein Labeling Kit | A20174 |
Alexa Fluor dye family
Illuminating research for over 15 years
Invitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.