The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range.
Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
We offer Alexa Fluor 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.
Alexa Fluor 647 dye dashboard
 Initial brightness |
Ideal for imaging low-abundance targets, especially in fixed cells; commonly used with Invitrogen Alexa Fluor 350, 488, and 594 dyes for multiplexing. In addition, is extremely useful in stochastic optical reconstruction microscopy (STORM) where it is an exceptional reporter for both dSTORM and nSTORM. It is also used in two-photon excitation (TPE) microscopy . | ||
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 Photostability in antifade |
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 Performance in SRM |
| 594/633 | Cy5 | 650 | 665 | ||
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Alexa Fluor 647 products
We offer a broad range of Alexa Fluor 647 products including labeling kits, secondary antibodies, bioconjugates, and more:
- Super-resolution microscopy (SRM) applications
- Antibody labeling kits for direct detection
- Primary antibodies for direct detection
- Secondary antibodies for flexible labeling
- Streptavidin conjugates for signal amplification
- Tyramide labeling kits for ultimate sensitivity
- Phalloidin conjugates for actin staining
- Annexin V conjugates for apoptosis imaging
- Hydrazides for neuronal tracing
- Labeled nucleotides for hybridization probes
STORM image of spectrin labeled with Alexa Fluor 647 in a hippocampal neuron. Image courtesy of Xue Liang Zhu et. al. Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai.
Alexa Fluor 647 reactive dyes
Select dye-labeling chemistries for conjugation with the following reactive groups:
Alexa Fluor 647 protein/antibody labeling kits
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Alexa Fluor 647 Mouse IgG1 Labeling Kit | Z25008 |
| <1–20 µg mouse IgG2a | Zenon Alexa Fluor 647 Mouse IgG2a Labeling Kit | Z25108 |
| <1–20 µg mouse IgG2b | Zenon Alexa Fluor 647 Mouse IgG2b Labeling Kit | Z25208 |
| <1–20 µg human IgG | Zenon Alexa Fluor 647 Human IgG Labeling Kit | Z25408 |
| <1–20 µg rabbit IgG | Zenon Alexa Fluor 647 Rabbit IgG Labeling Kit | Z25308 |
| Conventional antibody/protein labeling | ||
| 10–20 µg antibody | APEX Alexa Fluor 647 Antibody Labeling Kit | A10475 |
| 20–100 µg antibody/protein | Alexa Fluor 647 Microscale Antibody/Protein Labeling Kit | A30009 |
| 100 µg antibody/protein | Alexa Fluor 647 Antibody/Protein Labeling Kit | A20186 |
| 1 mg antibody/protein | Alexa Fluor 647 Antibody/Protein Labeling Kit | A20173 |
Alexa Fluor dye family
Illuminating research for over 15 years
Invitrogen Alexa Fluor dyes are among the most trusted fluorescent dyes available today. In addition to superior performance, you’ll get a technical support team experienced in problem-solving, >50,000 published references, application and experimental tips, and protocols to help with experimental planning. For an outstanding research experience, choose Alexa Fluor dyes.
- Learn more about the brightness of Alexa Fluor dyes
- Learn more about the photostability of Alexa Fluor dyes
- Read all about Alexa Fluor dyes in the Molecular Probes Handbook
Resources
Molecular Probes Handbook
Antifade reagents
Antifade reagents suppress photobleaching and preserve the signals of your fluorescently labeled target molecules. They offer excellent compatibility with a multitude of dyes across the spectrum, making them especially valuable tools for multicolor applications. For added convenience, some formulations include the nuclear counterstain DAPI, eliminating the need for a separate counterstaining step.
See the performance of this dye in SlowFade Antifade reagents
See the performance of this dye in ProLong Antifade reagents
Cell stain tool
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Fluorescence SpectraViewer
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.