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Multi-Well Dialysis Plates
Multi-well dialysis plates allow for microdialysis of many small volume samples (0.1–0.3 mL) concurrently and are available in varied molecular weight cutoffs (MWCOs). Also called microdialysis plates, multi-well dialysis plates are commonly used for a variety of protein sample cleanup purposes including protein and DNA desalting/buffer exchange, small molecule contaminates and unused reagent removal, and removal of dyes after protein labeling. These microdialysis plates are automation-compatible and feature regenerated cellulose membranes with a very large surface-area-to-volume ratio for dialysis experiments that take 2–4 hours to complete and help provide greater than 90% protein recovery.
Multi-well dialysis plates
We offer multi-well dialysis plates as 96-well dialysis plates and 48-well dialysis plates.
96-well dialysis plates
96-well dialysis plates are well suited for high-throughput runs of dialysis and can be automated using a liquid handling system.
48-well dialysis plates
48-well dialysis plates are well suited for mid-throughput runs of dialysis and can handle higher volume of samples up to 1 mL. They can also be automated using a liquid handling system.
Microdialysis plates selection guide
Recommended accessories
Adhesive-coated plastic plate seals are recommended to seal 96-well or 48-well microdialysis plates to help protect against contamination and evaporation, especially during long-term storage.
96-well deep-well plate, 2.2 mL—polypropylene deep-well assay plate is required for 96-well microdialysis devices; for 48-well microdialysis kits, the deep-well plate is included by default.
How to use microdialysis plates
Microdialysis multi-well plates are available in two formats: a 96-well format, which contains 12 strips with 8 microdialysis devices per strip, and a 48-well format, which contains 6 strips with 8 microdialysis devices per strip. Each device on the strip can be removed from the plate and separated into individual devices, permitting the flexibility to use only what is needed.
The workflow starts by removing the number of dialysis devices needed for your experiment, adding dialysis buffer to your deep-well plate or microcentrifuge tube, loading your sample into the microdialysis device, then placing the device into the plate prepared with buffer, dialyzing, and recovering your sample with a pipet (Figure 1). Dialysis time will vary depending on salt and molecular weight concentrations but typically complete in 2 to 4 hours.
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Figure 1. Workflow for Pierce 96-well Microdialysis Plates.
Efficiency and speed of 96-well microdialysis plates
Microdialysis devices are offered at 2K, 3.5K, 10K, and 20K MWCOs. Experimental data demonstrate that the rate of dialysis, as shown in conductivity in milliSiemens (mS), is similar for the various 100 μL microdialysis device MWCOs (Figure 2). When evaluating efficiency of timed dialysis changes, conductivity decreases significantly in just 30 minutes, demonstrating that the majority of low molecular weight compounds are removed from the samples (Figure 3).
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Figure 2. Similar rate of dialysis across different MWCOs—100 µL. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 30 minute intervals over a 2.5 hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.
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Figure 3. Comparing efficiency of timed dialysis changes—96 well. Samples of 0.1 mL (0.5 mg/mL Human IgG containing 1 M NaCl) were dialyzed against 1.8 mL of water in a 96 deep-well plate at room temperature. The water was changed at 15-, 30-, and 1-hour intervals over a 3-hour period. The rate of NaCl removal was determined by measuring the conductivity of the sample at the indicated time intervals.
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