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Citations & References (170)
Invitrogen™
Lipofectamine™ 2000 Transfection Reagent
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety ofRead more
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| Catalog Number | Quantity |
|---|---|
| 11668027 | 0.75 mL |
| 11668019 | 1.5 mL |
| 11668500 | 15 mL |
| 11668030 | 0.3 mL |
Catalog number 11668027
Price (USD)
545.65
Online Exclusive
590.00Save 44.35 (8%)
Each
Quantity:
0.75 mL
Price (USD)
545.65
Online Exclusive
590.00Save 44.35 (8%)
Each
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine™ 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
Green FeaturesSustainable packaging
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine
Product TypeTransfection Reagent
Quantity0.75 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Contains one vial (0.75 ml) Lipofectamine™ 2000 Reagent. Store at 2–8°C. Do not freeze.
Frequently asked questions (FAQs)
I transfected GFP into cells using Lipofectamine 2000 and saw a light granular orange background fluorescence. What could be causing this?
I accidentally left my lipid reagent at room temperature. Can I still use it?
Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA?
Can I use Lipofectamine 2000 to co-transfect plasmids and siRNA?
For how long is the Lipofectamine 2000:DNA complex stable?
Citations & References (170)
Citations & References
Abstract
Evidence that bovine forebrain embryonic zinc finger-like gene influences immune response associated with mastitis resistance.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16611727
Mastitis, a mammary gland inflammation in response to bacterial infection, is a major problem in the dairy industry. We found that cows susceptible to mastitis have a three-base insertion in a glycine-coding stretch of the gene for forebrain embryonic zinc finger-like (FEZL), a transcription factor with a role in neuronal
Characterization of aquaporin-6 as a nitrate channel in mammalian cells. Requirement of pore-lining residue threonine 63.
Journal:J Biol Chem
PubMed ID:12177001
Aquaporins (AQP) were originally regarded as plasma membrane channels that are freely permeated by water or small uncharged solutes but not by ions. Unlike other aquaporins, AQP6 overexpressed in Xenopus laevis oocytes was previously found to exhibit Hg2+ or pH-activated ion conductance. AQP6 could not be analyzed electrophysiologically in mammalian
Folding of the striated muscle myosin motor domain.
Journal:J Biol Chem
PubMed ID:12110670
'We have investigated the folding of the myosin motor domain using a chimera of an embryonic striated muscle myosin II motor domain fused on its COOH terminus to a thermal stable, fast folding variant of green fluorescent protein (GFP). In in vitro expression assays, the GFP domain of the chimeric
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19307578
'Nucleic acid reagents, including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammalian cells and are promising starting points for the development of new therapeutic agents. Realizing their full potential, however, requires nucleic acid delivery reagents that are simple to prepare, effective across many
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Journal:J Virol
PubMed ID:15767407
'Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both