Intelligently Simple AllType™ FASTplex™ NGS

Discover a simple, fast, and integrated solution for high-resolution genotyping!

Next Generation Sequencing (NGS) is a powerful technology offering high resolution typing with greater sample throughput. Nonetheless, many labs find it difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges.

Designed for simplicity and efficiency, our AllType FASTplex NGS Assay makes NGS accessible and achievable for any laboratory. The assay’s advanced chemistry and comprehensive coverage is complemented by a streamlined workflow for minimal hands-on time and supported by intuitive software for easy sample auditing and enhanced data integration.

Why Choose the AllType FASTplex NGS Solution?

  • One multiplex PCR reaction per sample
  • Early sample pooling
  • Total hands-on time <90 minutes
  • Automated data transfer and analysis

For Research Use Only. Not for use in diagnostic procedures.

| About the Assay

AllType FASTplex – Quality with Less Complexity

The AllType FASTplex chemistry has been enhanced to improve performance, robustness, and reliability. With the newly added Class II exon 1 primer mix, the AllType FASTplex reagents reduce ambiguities, provide comprehensive coverage across 11 loci, and generate highly concordant results at 99.8% or higher, all in a single PCR reaction.

Workflow Simplicity and Reduced Hands-On Time

The assay will enhance your lab’s efficiency with reduced labor, pipetting, and risk of contamination. By combining fragmentation and barcoding steps into a single reaction, the workflow allows for samples to be pooled early in the protocol so that library preparation can be completed in a single tube.

The protocol has been designed for simplicity, easy implementation, and greater cross-training in the lab. From start to finish, the workflow can be performed in under 7 hours with less than 90 minutes of hands-on time.

 

Library Preparation

< 7 hours total
< 90 minutes
hands-on time*

Sequencing

  • Ion GeneStudio S5​
  • Illumina MiniSeq
  • Illumina MiSeq
  • Illumina iSeq 100

Analysis

  • Automated withTypeStream VisualNGS Analysis Software

Reported & Advanced Analytics

  • Data integration withHistoTrac System &HLA Fusion Software

* Approximate time for a single tube library preparation protocol. Time to results may vary depending on sequencer and sequencing kit.

| About the NGS Instruments & Software

Streamlined Next Generation Sequencing Solution That's Flexible

When the workflow is complete, our TypeStream™ Visual NGS Analysis Software will begin the automatic analysis of your data while you’re away from your desk. The streamlined interface enables easy sample auditing, bi-directional communication with the SystemLink™ HistoTrac™ system as well as data migration into our HLA Fusion™ software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.

Platform-specific barcoding allows for sequencing on the Illumina™ MiSeq™, MiniSeq™, iSeq™ 100, Ion Chef™, and Ion GeneStudio™ S5 sequencing systems.

Intuitive Data Integration

For the first time, the TypeStream Visual software now includes bi-directional communication with the SystemLink™ HistoTrac™ System, enabling the seamless transfer of results between both systems for better data management, reduced transcription errors, and optimized reporting.

Your results can also be combined in our HLA Fusion™ Software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.

 

| About the End-to-End Support

Expert NGS Genotyping Support

As the leader in HLA for over 30 years, we provide comprehensive hands-on user training and unparalleled customer support. Our NGS Workshops introduce researchers to next-generation sequencing technology and allow users to practice the entire workflow. Our Field Applications Team offers expert NGS genotyping support for more advanced users. Our customers are our number one priority and we're proud to support the complete NGS workflow from reagents to instruments and software. We're here for you every step of the way.

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