AllType™ NGS Assays

The Quality You Expect and Simplicity You Deserve

Multiplex NGS Based Genotyping

Our portfolio of AllType™ assays offers a complete next generation sequencing solution that provides single tube amplification for all eleven Class I and Class II loci, completely removing the need for amplicon pooling. Samples are transferred directly to library preparation and made ready for sequencing in a single workday. Sequencing analysis and individual allele assignments are automatically generated by our TypeStream™ Visual NGS Analysis Software. Results are delivered in fewer than three days.

NGS technology designed for simplicity and efficiency

  • One multiplex PCR reaction per sample
  • Early sample pooling
  • Total hands-on time <90 minutes
  • Simple assay that anyone can learn

Multiplex PCR for Genotyping with NGS Offers a Simple, Fast, and Better Workflow

Many labs might find NGS difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges. Our NGS assays are designed for simplicity and flexibility allowing laboratories greater cross-training on their platform of choice.

Platform-specific barcoding allows for sequencing on the Illumina MiSeq, MiniSeq, iSeq™ 100, and Ion GeneStudio S5 sequencing systems.

 

Choose the Right NGS Assay, Protocol, and Coverage for Your Lab

Our AllType and AllType FASTplex NGS assays apply a single multiplex reaction to amplify all major Class I and Class II loci, completely removing the need for amplicon pooling. Both provide scalable solutions for low- and high-volume laboratories.

 


AllType FASTplex NGS

NGS is a powerful but complex technology genotyping. An intelligently simple approach, the One Lambda™ AllType FASTplex NGS Assay is the only easy, single-test solution supported by streamlined and integrated software.

Using our TypeStream™ Visual NGS Analysis Software, analysis of your results will begin automatically while you’re away from your desk. The interface has been upgraded for intuitive navigation and easy sample auditing. The analysis speed itself is scalable according to the needs of your lab.
 

Explore the AllType FASTplex Workflow

Library Preparation

< 7 hours total
< 90 minutes hands-on time*

Sequencing

  • Ion GeneStudio S5​
  • Illumina MiniSeq
  • Illumina MiSeq
  • Illumina iSeq 100

Analysis

  • Automated with TypeStream Visual NGS Analysis Software

Reported & Advanced Analytics

  • Data integration with HistoTrac System & HLA Fusion Software

* Approximate time for a single tube library preparation protocol. Time to results may vary depending on sequencer and sequencing kit.

 

  • Quality with less complexity
  • Workflow simplicity and reduced hands-on time
  • Streamlined data for automated analysis
  • Intuitive data integration

AllType NGS

Our classic AllType NGS assays offer a complete NGS solution that delivers unambiguous, high-resolution genotyping results in less than three days. This solution delivers single tube amplification for all eleven Class I and Class II loci, completely removing the need for amplicon pooling. Samples are transferred directly to library preparation and made ready for sequencing in a single workday.
 

Explore the AllType NGS Workflow

Library Preparation

< 8.5 hours total
< 3.5 hours
hands-on time*

Sequencing

  • Ion GeneStudio S5​
  • Illumina MiniSeq
  • Illumina MiSeq
  • Illumina iSeq 100

Analysis

  • Automated withTypeStream VisualNGS Analysis Software

Reported & Advanced Analytics

  • Data integration withHistoTrac System &HLA Fusion Software

* Time to results may vary depending on sequencer and sequencing kit.

  • Multiplexed amplification of Class I and Class II loci in only 2.5 hours
  • Compatible with multiple platforms
  • Results in less than 3 days
  • First-pass high resolution genotyping
It all starts with a demo
Click here to request a demo

 


Our Solutions

AllType™ FASTplex™ NGS
This assay combines the convenience of multiplex PCR with workflow simplicity by eliminating most of the pipetting, purification, and quantification steps.
AllType™ NGS
This single multiplex reaction amplifies all major Class I and Class II loci, completely removing the need for amplicon pooling.
TypeStream™ NGS Analysis Software
Perform genotyping from next-generation sequencing (NGS) data produced using the AllType NGS Typing Kits. Seamless integration with HLA Fusion Software.