LinkSēq™ PCR Typing Kits

Fast and Easy Real-time PCR Genotyping

LinkSēq is a rapid genotyping method with a simple workflow that prevents post-amplification contamination. With a fast turnaround time of only 90 minutes, and minimal hands-on time, your lab’s productivity will greatly increase.

With our LinkSēq kits, you can achieve high throughput. By using inexpensive thermal cyclers for the first 60 minutes of the amplification step and only 10 minutes on a real-time PCR instrument, you can type multiple samples per hour with unmatched simplicity. Our product line includes HLA genotyping of HLA and KIR, and blood genotyping of RBC, RH, ABO, and HPA

Learn more about our blood genotyping kits  ❯

 

LinkSēq Workflow – 90 Minutes to Results

  

LinkSeq workflow step 1 "add" graphic

Add
Hands On: 10 min

  

LinkSeq step 2 "amplify" graphic

Amplify
Hands off: 70 min

  

LinkSeq step 3 "analyze" graphic

Analyze
Automated: 10 min


LinkSēq HLA Typing Kits

Our LinkSēq reagents provide the most complete solution for high throughput laboratories interested in fast and easy typing of all 11 loci. This product provides low resolution typing for HLA A, B, C, DRB1, DRB345, DQA1, DQB1, DPA1, and DPB1, as well as common null alleles.

Information
  • Molecular detection features SYBR Green Real-Time PCR
  • All necessary reagents are included in the kit
  • No contamination control needed
  • Less than 10 minutes of hands-on
  • Genotyping results of Class I and II loci in 90 minutes
  • Fewer repeats, faster workflow steps, and reproducible results

LinkSēq HLA - Specific Genetic Markers

These kits type specific HLA alleles such as B27 or B*57:01. Each kit types a few specific alleles in strip format. This is the fastest and most cost effective solution for labs looking for specific typing of the alleles included in the strip.

  • Typing of specific HLA alleles in strip format

LinkSēq KIR Typing Kits 

Killer cell immunoglobulin-like receptors (KIR) are a family of cell surface receptors found on human Natural Killer (NK) cells. Certain KIR types interact with HLA Class I molecules to inhibit the activity of NK cells while other KIR types can stimulate NK activity. The LinkSēq KIR typing test types all 15 KIR genes and 2 pseudogenes including both full length and deleted forms of 3DP1 and 2DS4 and allele-specific variants in 3DL1, 2DL1, and others.

Information
  • Available in 96- and 384-well trays.
  • All necessary reagents included in kit.

 

  • No contamination control needed
  • Less than 10 minutes of hands-on
  • Genotyping results of Class I and II loci in 90 minutes
  • Fewer repeats, faster workflow steps, and reproducible results

LinkSēq Ancillary

Our accessories support the use of the core LinkSēq products and a key ancillary product is the LinkSēq wipe test. According to widely accepted Standards and Guidelines (e.g. ASHI and EFI), laboratories must routinely monitor for contamination of pre-amplification areas by the most common amplification products that are produced in the laboratory.

The LinkSēq Wipe Test was developed to detect both genomic DNA contamination as well as potential LinkSēq amplicon contamination. Additional products include accessories to ensure that your needs are covered.

  • Detects both genomic DNA contamination as well as potential LinkSēq amplicon contamination
It all starts with a demo
Click here to request a demo for LinkSēq™ products