Sequencing in a Single Workday AllType™ NGS Multipexing
NGS Based Genotyping with Fast Turnaround From the Leader in HLA
The AllType all-in-one reaction reduces the probability of error, eliminates the need for sample prep automation, and offers additional advantages in efficiency, ease-of-use, and overall turnaround time. AllType™ NGS assays offer a complete NGS solution that delivers unambiguous, high-resolution genotyping results in less than three days.
- One multiplex PCR reaction per sample
- No amplicon pooling; direct to library preparation
- Multiplex amplification of all Class I and Class II loci in only 2.5 hours
- Suitable for low and high volume laboratories
Multiple regulatory designations. View individual products.
Why Choose the AllType FASTplex NGS Solution?
Streamlined Next Generation Sequencing Solution
Our AllType NGS Assay represents the latest in long-range multiplex PCR technology. By amplifying all major Class I and Class II loci in a single PCR reaction, AllType completely removes the need for amplicon pooling and allows samples to be transferred directly to library preparation and made ready for sequencing in a single workday.
The AllType workflow offers the flexibility of platform-specific barcoding to allow for sequencing on the Ion GeneStudio S5 and Illumina MiSeq platforms. The speed and automation of the Ion Chef and GeneStudio S5 System delivers efficiency and reliability while TypeStream™ Visual, our state-of-the-art data analysis software, automates genotyping to further shorten time-to-results.y.
The AllType NGS 11-Loci Amplification Kit amplifies all eleven Class I and Class II loci in a single tube multiplex reaction in only 2.5 hours. The AllType workflow application is completely platform flexible and allows for sequencing using barcode adapters specific for your platform of choice.
AllType final library pools are labeled using Ion Xpress barcodes and loaded onto the Ion Chef System on day one. Isothermal amplification, enrichment and chip-loading is completed overnight and sequenced the following morning on the Ion GeneStudio S5 System. Sequencing and base calling is completed the same day in less than 5 hours from start to finish.
- Shorten instrument setup time to < 5 minutes
- Automated template preparation in < 7 hours
- Complete sequencing in < 5 hours
- Report genotyping results in < 3 days
- Suitable for low and high volume laboratories
- Shorten time to results with automated genotyping
- Get One Lambda front line support for the entire workflow
AllType final library pools are labeled using AllType Index Flex barcodes, loaded onto the Illumina MiSeq on day one and run overnight. Bridge amplification, sequencing and base calling is accomplished on the Illumina MiSeq in a period of approximately 24 hours from start to finish.
- Shorten instrument setup time to < 15 minutes
- Automated onboard template preparation
- Complete sequencing in < 24 hours
- Report genotyping results in < 3 days
- Process up to 48 samples in a single run
TypeStream™ Visual (TSV) is a new NGS software solution that offers fully automated analysis of both single read and paired-end sequencing data, giving it the flexibility to analyze input from either major NGS platform.
The TSV software suite includes a wide range of analytical tools, run statistics and quality metrics that facilitates examination and reinforces decision making.
- Fully automated analysis
- Supports analysis of single read and paired-end sequencing data
- No network access required
- Data integration with HLA Fusion™ Research combines high resolution genotyping with: Sample Records, Antibody Tracking, Combined Analysis, Reporting
- Genotyping and variant calling algorithms exceed 99.7% concordance
Expert NGS Genotyping Support
As the leader in HLA for over 30 years, we provide comprehensive hands-on user training and unparalleled customer support. Our NGS Workshops introduce researchers to next-generation sequencing technology and allow users to practice the entire workflow. Our Field Applications Team offers expert NGS genotyping support for more advanced users. Our customers are our number one priority and we're proud to support the complete NGS workflow from reagents to instruments and software. We're here for you every step of the way.
Educational Resources about AllType NGS
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