The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:
• A strong, easily detectable FITC signal indicating transfection efficiency • Chemical modifications for a clearer, more persistent cellular signal than labeled siRNA provides • Proven correlation of transfection efficiency with Stealth RNAi™ siRNA and traditional siRNA molecules
This makes BLOCK-iT™ Fluorescent Oligo an ideal indicator of transfection efficiency for RNAi experiments. In addition, the kit contains a Positive Control Stealth RNAi™ siRNA molecule directed at human p53 and a Scrambled Stealth RNAi™ siRNA molecule as a negative control. To assay cell viability, Dead Cell Dye is included. This dye colors dead cells as red, thereby allowing easy identification of non-viable cells in a tissue culture plate. Using these reagents together allows you to both optimize transfection conditions and have a functional positive control in every RNAi experiment you perform.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Control Type
Cell Viability Control, Negative Control, Transfection Control, Positive Control