DNase I (Deoxyribonuclease I) digests single- and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. Ribonuclease has been reduced to non-detectable levels.

Applications
DNase I is suitable for removing DNA from protein preparations, nick translating DNA, and generating random fragments for dideoxy sequencing.

NOTE: for removing DNA from RNA preparations, use Amplification Grade DNase I (Cat. No. 18068-015).

Source
DNase I is purified from bovine pancreas.

Specific activity
The specific activity of DNase I is typically in the range of 10,000–25,000 units/mg.