Invitrogen™

Silencer™ siRNA Transfection II Kit

Catalog number: AM1631
Invitrogen™

Silencer™ siRNA Transfection II Kit

Catalog number: AM1631
Catalog Number
AM1631
Unit Size
1 kit
Price (USD)
Price: 754.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM16311 kit
Price: 754.00
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
This Ambion™ kit is for the optimization of transfection protocols for specific cell types. It includes 0.4 mL of each of two transfection agents; siPORT™ Amine Transfection Agent and siPORT™ NeoFX™ Transfection Agent along with control siRNAs.
• Broad cell line compatibility-kit includes siPORT™ Amine and siPORT™ NeoFX™ transfection agents
• Faster protocol optimization-kit includes ready-to-transfect GAPDH and negative siRNA controls The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, the Silencer™ siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. The Silencer™ siRNA Transfection II Kit includes siPORT™ Amine Transfection Agent (a polyamine mixture) and the new siPORT™ NeoFX™ Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types. Furthermore, both can be used to transfect cells during subculturing-saving a full day of valuable time-without increased cytotoxicity. In addition to containing a choice of transfection agents, this kit includes a well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies. A scrambled, negative control siRNA is also provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Control Type
Negative Control, Positive Control
Cell Type
Mammalian
Format
Kit
Sample Type
Synthetic siRNA, miRNA
Transfection Technique
Lipid-based Transfection
For Use With (Application)
siRNA Transfection
Product Line
Silencer™, Ambion™
Product Type
siRNA Transfection Optimization Kit
Quantity
1 mL
RNAi Type
siRNA

Contents & Storage

siPORT™ NeoFX™ Transfection Agent and siPORT™ Amine Transfection Agent should be stored at 4°C. GAPDH siRNA and Negative Control siRNA should be stored at –20°C.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text