Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

Catalog number: C640003
Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

Catalog number: C640003
Catalog Number
C640003
Unit Size
5 x 100 µL
Price (USD)
Price: 425.00
Online price: 395.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C6400035 x 100 µL
Price: 425.00
Online price: 395.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA, making MegaX DH10B T1R cells ideal for highly demanding cloning or cDNA and gDNA library construction applications.

The DH10B T1R strain has all the benefits of DH10B genotype, with the addition of the tonA (also known as fhuA) genotype that confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype offers added security to protect valuable clones and libraries. MegaX DH10B T1R cells carry mutations in the methylation-dependent E. coli K12 restriction system (mcrA, mcrBC, and mrr) allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has the lacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B T1R an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

MegaX DH10B T1R Electrocomp Cells offer:
• Highest transformation efficiency of >3 x 1010 cfu/μg
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
endA1 mutation enables increased plasmid yield and quantity
recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Note: A high-voltage electroporation apparatus is required.

Genotype
FmcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λrpsL(StrR) nupG tonA

Find the strain and format that fits your needs
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
Electroporation cuvettes are available in 0.1 cm, 0.2 cm, and 0.4 cm gaps.
Strains are available in several MultiShot formats for high throughput applications.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Electrocompetent Cells
Contains F' Episome
No
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
High Efficiency (>3 x 1010 cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Cloning Unstable DNA
Not suitable for cloning unstable DNA
Blue/White Screening
Yes (lacZΔM15)
High-throughput Compatibility
Low
Plasmid
May be used for plasmids >100 kb
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
Yes
Species
E. coli (K12)
Format
Tube
Product Line
Electrocomp™
Quantity
5 x 100 μL

Contents & Storage

• MegaX DH10B T1R Electrocomp Cells (5 x 100 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (50 μL)
Store pUC19 DNA at –20°C.

• Recovery Medium (3 x 10 mL)
Store Recovery Medium at 4°C

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text