Invitrogen™

TOP10 Electrocomp™ Kit

Catalog number: C66455
Invitrogen™

TOP10 Electrocomp™ Kit

Catalog number: C66455
Catalog Number
C66455
also known as C664-55
Unit Size
5 x 100 µL
Price (USD)
Price: 303.00
Your price:
Availability
-
Quantity
Catalog Number
C66411
also known as C664-11
Unit Size
10 x 100 µL
Price (USD)
Price: 538.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C66455
also known as C664-55
5 x 100 µL
Price: 303.00
Your price:
-
C66411
also known as C664-11
10 x 100 µL
Price: 538.00
Your price:
-
Product Overview
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TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids. 

The TOP10 genotype is similar to the DH10B strain and features:
hsdR for efficient transformation of unmethylated DNA from PCR amplifications
mcrA for efficient transformation of methylated DNA from genomic preparations
lacZΔM15 for blue/white color screening of recombinant clones
endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
recA1 for reduced occurrence of non-specific recombination in cloned DNA

Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

Find the strain and format that fits your needs
TOP10 is available in chemically competent and electrocompetent cell formats.
Choose MultiShot competent cells for high-throughput cloning applications.
Email us for more information about custom formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Electrocompetent Cells
Contains F' Episome
No
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
High Efficiency (>1 x 1010 cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Cloning Unstable DNA
Not suitable for cloning unstable DNA
Blue/White Screening
Yes (lacΔM15)
High-throughput Compatibility
Low
Plasmid
May be used for plasmids >20 kb
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli (K12)
Format
Tube
Product Line
Electrocomp™
Quantity
5 x 100 μL

Contents & Storage

• 5 x 100μL TOP10 Electrocomp E. coli
• 50 μL pUC19 vector (10 pg/μL)
Store at –80°C.

• S.O.C. Medium (15 mL)
Store at room temperature.

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