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Catalog Number
F10473
Unit Size
1 x 1 L
Price (USD)
Price: 5,140.00
Online price: 5,088.65
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Unit Size
10 x 10 mL
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Price: 672.00
Online price: 667.65
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Unit Size
1 x 100 mL
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Price: 624.00
Online price: 618.65
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Product Overview
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The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence
This advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence
This advanced formulation allows the assay to be run in a simple “addition only” format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Green
Detection Method
Fluorescent
For Use With (Application)
Cell Viability and Proliferation
For Use With (Equipment)
Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
Product Type
Calcium Assay Kit
Dye Type
Fluorescent Dye-Based
Emission
Visible
Form
Powder
Modification
Chemical
Product Line
Molecular Probes™
Quantity
1000 ml
Shipping Condition
Room Temperature
Sub Cellular Localization
Nucleus, Organelles, Cytoplasm
Contents & Storage
- 1 L Fluo-4 Direct™ Calcium Assay Reagent (Component A)
- 1.54 g Probenecid (Component B)
- Store at ≤-20°C. Dessicate and protect from light.
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