5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression.
The Thermo Scientific EpiJET DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyze DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5'-CCGG-3' tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected.

The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Highlights

• Fast—complete digestion of genomic DNA in 1 hour at 37°C
• Efficient—specially formulated enzymes for epigenetic studies of DNA methylation status

Applications

• CpG methylation analysis at 5'-CCGG-3' loci

Includes

• Epi Mspl: 200 μL
• Epi HpaII: 200 μL
• 10X EpiBuffer: 1 mL
• Control pUC19/SmaI DNA Unmethylated (0.5 μg/ μL): 20 μL
• Control pUC19/SmaI DNA CpG Methylated (0.5 μg/ μL): 20 μL

Note

DNA sample quality is important for efficient digestion by restriction endonucleases or downstream applications. We recommend use of spin column based kits, such as the Thermo Scientific GeneJET Genomic DNA Purification Kit (#K0721, #K0722).