GeneBLAzer™ H1-NFAT-bla HEK293T cells contain the human Histamine Receptor 1 (H1) stably integrated into the CellSensor™ NFAT-bla HEK293T cell line. CellSensor™ NFAT-bla HEK293T cells (Cat. no. K1538) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.
The GeneBLAzer™ H1-NFAT-bla HEK293T cells are functionally validated for Z' and EC50 concentrations Histamine. In addition, GeneBLAzer™ H1-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
The H1 receptor is located in human bronchi, bronchioles, and bronchial muscle, smooth muscle of the ileum, and the uterus. H1 receptors play a role in various disorders, including allergic reactions and mood disorders.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_000861.3
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
H1
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
HRH1, H1
Type
Cell Line
Cell Line
293T (HEK)
System Type
GeneBLAzer™
Druggable Target
GPCR
Contents & Storage
Contents: K1703 GeneBLAzer™ H1-NFAT-bla HEK293T cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ H1-NFAT-bla HEK293T cells • GeneBLAzer™ H1-NFAT-bla HEK293T Assay Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ H1-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.