GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells contain the human Purinergic receptor P2, G protein-coupled, 11 (P2RY11) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. no. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.
The GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of ATP. In addition, GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
P2RY11 expression is broadly distributed and has been found in the brain, pituitary, lymphocytes, spleen, intestines, macrophages, lung, stomach, adipose tissue, pancreas, kidney, prostate, heart, placenta, liver, and skeletal muscle. Differentiation of human promyelocytic HL60 cells and maturation of monocyte-derived dendritic cells have been shown to be mediated by ATP activation of P2RY11.
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For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession Number
NM_002566.4
Assay Entry
Cell-based beta lactamase reporter gene
Cell State
Dividing Cells
Detection Method
Fluorescent
For Use With (Application)
FRET
Gene Symbol
P2RY11
Population Status
Single Cell Clone Origin
Product Line
GeneBLAzer™
Reporter Gene
BLA (Beta-Lactamase)
Target Entry
P2RY11
Cell Line
CHO-K1
System Type
GeneBLAzer™
Druggable Target
GPCR
Contents & Storage
Contents: K1729 GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells (LiveBLAzer™-FRET B⁄G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells • GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis
Storage: The GeneBLAzer™ P2RY11-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.