Informational:New Protein Expression Support Center—find Mammalian Expression technical tips and troubleshooting recommendations from our scientists.
Informational:Get your genes cloned into this vector with GeneArt Gene Synthesis Services
Invitrogen™

pcDNA™3.3-TOPO™ TA Cloning™ Kit

Catalog number: K830001
Invitrogen™

pcDNA™3.3-TOPO™ TA Cloning™ Kit

Catalog number: K830001
Catalog Number
K830001
also known as K8300-01
Unit Size
20 reactions
Price (USD)
Price: 1,690.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
K830001
also known as K8300-01
20 reactions
Price: 1,690.00
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The pcDNA™3.3-TOPO™ TA Cloning™ Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.3™-TOPO™ TA Cloning™ Kit is supplied with the TOPO™-adapted plasmid vector, all reagents for cloning, and One Shot™ TOP10 competent cells. The pcDNA™3.3-TOPO™ vector offers the following benefits:

• TOPO™ adapted for cloning PCR products with >85% efficiency
• High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp)
• Vector is ideal for large-scale protein expression
• Generation of native protein without extraneous amino acids

The pcDNA™3.3-TOPO™ vector is also included with the OptiCHO™ Antibody Express System for cloning and expression of light and heavy antibody chains.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
CMV
Product Type
TOPO TA Cloning Kit
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
Untagged
Cloning Method
TOPO-TA
Vector
pcDNA
Product Line
TOPO™, pcDNA™
For Use With (Application)
Protein Production, Constitutive Expression

Contents & Storage

Box 1 (store at -20°C)
• 20 μl pcDNA™3.3-TOPO™ vector, TOPO™ adapted (5–10 ng/μl)
• 100 μl 10X PCR Buffer
• 10 μl dNTP Mix
• 50 μl Salt Solution
• 1 ml Sterile Water
• 10 μl Control PCR Template (50 ng/μl)
• 10 μl Control PCR Primers (100 ng/μl each)
• 20 μl CMV Forward Sequencing Primer (100 ng/μl)
• 20 μl TK polyA Reverse Sequencing Primer (100 ng/μl)
• 10 μl pcDNA™3.3-TOPO™/lacZ Expression Control Plasmid (500 ng/μl)

Box 2 (store at -80°C)
• 11 × 50 μl TOP10 E. coli
• 50 μl pUC19 Control DNA (10 pg/μl)
• 6 ml S.O.C. Medium

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text