Informational:Please note that at certain times of the year Bis-Tris protein gel boxes may arrive inside insulated coolers containing ambient temperature gel packs.
Invitrogen™

NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Catalog number: NP0321BOX
Invitrogen™

NuPAGE™ 4 to 12%, Bis-Tris, 1.0–1.5 mm, Mini Protein Gels

Catalog number: NP0321BOX
Invitrogen NuPAGE Bis-Tris protein gels are precast polyacrylamide gels that provide broad molecular weight protein separation with high resolution and sample integrity. These precast gels are ideal for applications where protein integrity is crucial. Unlike traditional Tris-glycine gels, NuPAGE Bis-Tris gels have a neutral pH environment that minimizes protein modifications. Use NuPAGE Bis-Tris gels for preparing proteins for sequencing, mass spectrometry, and any other techniques where protein integrity is important.
 
Catalog Number
NP0321BOX
Unit Size
10 gels (1 box)
Wells
10-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
2 gels (1 box)
Wells
10-well
Gel Thickness
1.0 mm
Quantity
2 Gels/Box
Price (USD)
Price: 35.53
Online price: 34.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
12-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volumeup to 20 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
2 gels (1 box)
Wells
12-well
Gel Thickness
1.0 mm
Quantity
2 Gels/Box
Price (USD)
Price: 35.53
Online price: 34.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volumeup to 20 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
15-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
2 gels (1 box)
Wells
15-well
Gel Thickness
1.0 mm
Quantity
2 Gels/Box
Price (USD)
Price: 35.53
Online price: 34.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
1-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume700 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells1-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
2D-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 185.00
Online price: 180.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells2D-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
9-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 28 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells9-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Catalog Number
Unit Size
10 gels (1 box)
Wells
17-well
Gel Thickness
1.0 mm
Quantity
10 Gels/Box
Price (USD)
Price: 162.00
Online price: 157.65
Your price:
Availability
-
Full specifications
Quantity
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells17-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 10 of 16Show more
Catalog NumberSpecificationsUnit SizeWellsGel ThicknessQuantityPrice (USD)AvailabilityQuantity
NP0321BOXFull specifications
10 gels (1 box)10-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0321PK2Full specifications
2 gels (1 box)10-well1.0 mm2 Gels/Box
Price: 35.53
Online price: 34.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells10-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
NP0322BOXFull specifications
10 gels (1 box)12-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volumeup to 20 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0322PK2Full specifications
2 gels (1 box)12-well1.0 mm2 Gels/Box
Price: 35.53
Online price: 34.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volumeup to 20 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells12-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
NP0323BOXFull specifications
10 gels (1 box)15-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0323PK2Full specifications
2 gels (1 box)15-well1.0 mm2 Gels/Box
Price: 35.53
Online price: 34.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells15-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity2 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size2 gels (1 box)
NP0324BOXFull specifications
10 gels (1 box)1-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading Volume700 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells1-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0326BOXFull specifications
10 gels (1 box)2D-well1.0 mm10 Gels/Box
Price: 185.00
Online price: 180.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells2D-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0327BOXFull specifications
10 gels (1 box)9-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 28 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells9-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
NP0329BOXFull specifications
10 gels (1 box)17-well1.0 mm10 Gels/Box
Price: 162.00
Online price: 157.65
Your price:
-
For Use With (Equipment)Mini Gel Tank, XCell SureLock Mini-Cell
Gel Percentage4 to 12%
Gel SizeMini
Gel Thickness1.0 mm
Gel TypeBis-Tris
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 15 μL
Separation Range3.5 to 260 kDa
Separation TypeDenaturing
Shelf Life12 Months
Wells17-well
Mode of SeparationMolecular Weight
Product LineNuPAGE™
Quantity10 Gels/Box
Shipping ConditionRoom Temperature
Storage RequirementsStore at 4–25°C. Do not freeze.
Length (Metric)8 cm
Width (Metric)8 cm
Unit Size10 gels (1 box)
Showing 10 of 16Show more

Features of NuPAGE Bis-Tris gels:
Better protein integrity—optimized sample preparation process preserves your proteins
Wide ranges of molecular weight separation—select the right gel and running buffer to get the optimal separation of your proteins
Faster run times—get separation of your proteins in as little as 35 minutes
Longer shelf life—NuPAGE Bis-Tris gels can be stored for at least 12 months at room temperature

Choose the right NuPAGE Bis-Tris gel for your protein separation
Obtain optimal separation of your proteins by choosing the right combination of gel and running buffer. NuPAGE Bis-Tris protein gels come in four polyacrylamide concentrations: 8%, 10%, 12%, and a 4–12% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Bis-Tris gels also come in multiple well formats.

NuPAGE Bis-Tris gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, use the NuPAGE LDS Sample Buffer (NP0007) and NuPAGE Sample Reducing Agent (NP0004). Use NuPAGE Antioxidant (NP0005) in the running buffer to maintain the reduced state of the proteins during the run and to allow maximum band sharpness. The gels can be run using NuPAGE MES SDS Running Buffer (NP0002) to better resolve small proteins or NuPAGE MOPS SDS Running Buffer (NP000102) to resolve medium- to large-size proteins.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP00061) for traditional wet transfer using the Mini Blot Module (B1000) or XCell II Blot Module (EI9051). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text