Informational:Informational:Save up to 31% on your order of cell culture products. View promotion details »
Gibco™

Ham's F-12 Nutrient Mix

Catalog number: 11765062
Gibco™

Ham's F-12 Nutrient Mix

Catalog number: 11765062
Catalog Number
11765062
also known as 11765-062
Unit Size
10 x 500 mL
Price (USD)
Price: 315.00
Online price: 289.65
Your price:
Availability
-
Quantity
Catalog Number
11765054
also known as 11765-054
Unit Size
500 mL
Price (USD)
Price: 36.45
Online price: 33.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 mL
Price (USD)
Price: 56.75
Online price: 53.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
6 x 1,000 mL
Price (USD)
Price: 303.00
Online price: 279.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
11765062
also known as 11765-062
10 x 500 mL
Price: 315.00
Online price: 289.65
Your price:
-
11765054
also known as 11765-054
500 mL
Price: 36.45
Online price: 33.65
Your price:
-
117650471,000 mL
Price: 56.75
Online price: 53.65
Your price:
-
117650706 x 1,000 mL
Price: 303.00
Online price: 279.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Ham's F-12 is modified as follows:
With
• L-glutamine
• Phenol Red


The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Cell Line
CHO, COS-7, and rat prostate epithelial cells
Cell Type
Primary Rat Astrocytes
Classification
Animal Origin-free
Concentration
1 X
Form
Liquid
Product Type
Ham's F-12 Nutrient Mixture
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
Glutamine, Phenol Red, Sodium Pyruvate
Without Additives
No HEPES
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
10 x 500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text