Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
---|---|---|---|---|---|
11765062 also known as 11765-062 | 10 x 500 mL | Price: 315.00 Online price: 289.65 Your price: | - | ||
11765054 also known as 11765-054 | 500 mL | Price: 36.45 Online price: 33.65 Your price: | - | ||
11765047 | 1,000 mL | Price: 56.75 Online price: 53.65 Your price: | - | ||
11765070 | 6 x 1,000 mL | Price: 303.00 Online price: 279.65 Your price: | - |
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With |
• L-glutamine |
• Phenol Red |
The complete formulation is available.
Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.