Gibco™

DMEM, high glucose, HEPES

Catalog number: 12430112
Gibco™

DMEM, high glucose, HEPES

Catalog number: 12430112
Catalog Number
12430112
also known as 12430-112
Unit Size
5 L
Price (USD)
Availability
-
Quantity
-
Catalog Number
12430054
also known as 12430-054
Unit Size
500 mL
Price (USD)
Price: 46.98
Online price: 41.65
Your price:
Availability
-
Quantity
Catalog Number
12430062
also known as 12430-062
Unit Size
10 x 500 mL
Price (USD)
Price: 404.00
Online price: 358.65
Your price:
Availability
-
Quantity
Catalog Number
12430047
also known as 12430-047
Unit Size
1,000 mL
Price (USD)
Price: 75.50
Online price: 69.65
Your price:
Availability
-
Quantity
Catalog Number
12430104
also known as 12430-104
Unit Size
6 x 1,000 mL
Price (USD)
Price: 396.00
Online price: 344.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
12430112
also known as 12430-112
5 LContact Us ›--
12430054
also known as 12430-054
500 mL
Price: 46.98
Online price: 41.65
Your price:
-
12430062
also known as 12430-062
10 x 500 mL
Price: 404.00
Online price: 358.65
Your price:
-
12430047
also known as 12430-047
1,000 mL
Price: 75.50
Online price: 69.65
Your price:
-
12430104
also known as 12430-104
6 x 1,000 mL
Price: 396.00
Online price: 344.65
Your price:
-
Product Overview
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine 
• HEPES 
• Phenol Red 


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Form
Liquid
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Quantity
5 L

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