The Algimatrix™ 3D Culture System provides a three-dimensional matrix that has the potential to create cell culture conditions that more closely reflect normal cell morphology and behavior. It has relevance for both academic and industrial researchers who want to culture hepatocytes and other spheriod forming cells. Algimatrix™ alginate sponge is provided in a 24 well format that fits well into the workflow of both lower and higher-throughput users. The larger well size enables growth of more cells for downstream applications such as transfection.