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Invitrogen™

Proteinase K, recombinant

Catalog number: 25530031
Invitrogen™

Proteinase K, recombinant

Catalog number: 25530031
Catalog Number
25530031
also known as 25530-031
Unit Size
1 g
Price (USD)
Price: 1,284.00
Online price: 1,175.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
100 mg
Price (USD)
Price: 176.00
Online price: 156.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
25530031
also known as 25530-031
1 g
Price: 1,284.00
Online price: 1,175.65
Your price:
-
25530015100 mg
Price: 176.00
Online price: 156.65
Your price:
-
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Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Proteinase K remains active:
• Over a wide pH range–optimal activity between 6.5 and 9.5
• Under denaturing conditions–e.g., in the presence of SDS or urea
• In the presence of metal chelating agents—e.g., EDTA
• At comparatively high temperatures–optimum digestion temperature is 65°C

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within 1 min at 37°C using hemoglobin as a substrate.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
>40 U/mg
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice
Quantity
1 g
Product Type
Proteinase K
Form
Powder

Contents & Storage

One tube Proteinase K
Store in refrigerator (2–8°C).

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