Informational:This product will cease production as of October 20th 2023. To continue with this content, contact your local sales representative and order TaqMan™ OpenArray™ Real-Time PCR Plate with Inventoried Gene Expression Assays format 224, Cat #4470201, with this product target list. Stock is anticipated to last into 2024 to support transition to custom plate manufacturing.
Applied Biosystems™
QuantStudio™ 12K Flex OpenArray™ Human miRNA Starter Kit
A variety of starter kits are available to introduce you to the various applications of the QuantStudio™ 12K Flex OpenArray™ sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The miRNA kit also contains cDNA controls to serve as samples for your experiments. Note that the QuantStudio™ 12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.
The accessories provided with this kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Description
Use high-quality total RNA samples containing miRNA.
Detection Method
Primer-probe
Format
384-Well Plate
PCR Method
2-step RT-qPCR
Polymerase
AmpliTaq Gold DNA Polymerase
Reaction Speed
Standard
For Use With (Equipment)
QuantStudio™ 12k Flex
Product Line
OpenArray™, QuantStudio™
Product Type
12K Flex Human miRNA Starter Kit
Reverse Transcriptase
MultiScribe™
For Use With (Application)
Pre-amplification, Pre-Amplification
Hot Start
Built-In Hot Start
Sample Type
Total RNA
Contents & Storage
The QuantStudio™ 12K Flex OpenArray™ Human miRNA Starter Kit contains: • TaqMan™ MicroRNA Reverse Transcription Kit (200 reactions) • Megaplex™ RT Primers, Human Pool A v2.1 • Megaplex™ PreAmp Primers, Human Pool A v2.1 • 2X TaqMan™ PreAmp Master Mix Kit • Human brain and lung total RNA, 100 μg (1 mg/mL) each • 2X TaqMan™ OpenArray™ Real-Time PCR Master Mix, 1.5 mL • TaqMan™ OpenArray™ Human MicroRNA Panels (2 plates) • QuantStudio™ 12K Flex OpenArray™ Accessories Starter Kit Upon receipt, store frozen unopened kit at -20°C.