This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with colorimetric detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using an Avidin-HRP (horseradish peroxidase) and DAB (3, 3'-Diaminobenzidine) substrate reaction. This kit has been optimized for IHC with both frozen and paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.

Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Microscopy, Immunocytochemistry, Immunohistochemical Staining of Frozen Tissue Sections