Applied Biosystems™

MicroSEQ™ ID Purification Combo Kit v2.0, with 8-strips kit

Catalog number: A35854
Applied Biosystems™

MicroSEQ™ ID Purification Combo Kit v2.0, with 8-strips kit

Catalog number: A35854
Catalog Number
A35854
Unit Size
1 kit
Price (USD)
Price: 308.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A358541 kit
Price: 308.00
Your price:
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The MicroSEQ ID Purification Combo Kit v2.0 includes reagents for post-PCR and post-sequencing clean-up in the MicroSEQ ID microbial identification workflow. This version contains one tube of ExoSAP-IT Express Reagent for post-PCR clean-up and 12 x 8-strips of MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit for post-sequencing clean-up.

ExoSAP-IT Express Reagent removes unincorporated dNTPs and primers from the post-amplification PCR mix in 5 minutes compared to 30 minutes with the original ExoSAP-IT Reagent while producing equivalent results. The MicroSEQ ID Ultra Sequencing Clean-up kit in 96-well plate or 8-strips format provides a convenient and simple way to remove dye terminators, dNTPs, salts, and residual primers from cycle sequencing reactions. The end products can be used directly for sequencing on any Applied Biosystems genetic analyzer.

Comparability studies demonstrated equivalent results between the original ExoSAP-IT Reagent and the improved ExoSAP-IT Express Reagent coupled with the MicroSEQ ID Ultra Sequencing Clean-Up kit in the MicroSEQ ID workflow (see figure below). Both ExoSAP-IT and ExoSAP-IT Express enzyme-treated PCR samples produced very similar sequencing results and the correct identification result.

ExoSAP-IT Express Reagent can be used downstream of the following PCR amplification kits:
• MicroSEQ 500 16S rDNA PCR Kit
• MicroSEQ Full Gene 16S rDNA PCR Kit
• MicroSEQD2 rDNA Fungal PCR Kit

MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit can be used downstream of the following sequencing kits:
• MicroSEQ 500 16S rDNA Sequencing Kit
• MicroSEQ Full Gene 16S rDNA Sequencing Kit
• MicroSEQ D2 rDNA Fungal Sequencing Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Strip
Sample Type
Bacteria, DNA, dsDNA, Fungi
For Use With (Application)
Sequencing, PCR
High-throughput Compatibility
High-throughput Compatible, Manual Protocols
No. of Reactions
100 Reactions
Product Line
MicroSEQ™
Shipping Condition
Approved for shipment on Wet or Dry Ice
Target
Fluorescent Dye Removal, DNA from Enzymatic Reactions (e.g. PCR)

Contents & Storage

• 1 x ExoSAP-IT Express Reagent (100 reactions). Store at -5 to -30°C.
• 1 x MicroSEQ ID Ultra Sequencing Clean-up 8-Strips Kit (12 x 8 strips). Store at 2–8°C.

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