Thermo Scientific 10X Buffer B ensures the optimum reaction conditions for specific restriction enzymes and is premixed with BSA for enhanced stability. It is part of Thermo Scientific Five Buffer System which ensures the optimum reaction conditions for each restriction enzyme. The system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme. AarI and BveI require an oligonucleotide (also supplied with the enzyme), and Esp3I requires DTT.