Suspension array analysis is a powerful technique enabling the simultaneous detection and quantitation of multiple analytes with a single sample. Using color coded microspheres with the appropriate dyes, these tests can now be carried out using commonly available flow cytometers.

• Cyto-Plex™ microspheres consist of a highly uniform polystyrene particle with fluorescent intensities which are completely separated from each other
• Carboxylated particles provide different sizes and levels of fluorescent intensities for analysis of multiple analytes
• Use of a single diameter particle for all dye levels saves time by only requiring the development and optimization of one bead chemistry; multiple diameters can be combined to increase the number of analytes measured in one test
• High-density binding sites and low non-specific binding enable coupling with a wide variety of antibodies, nucleic acids and other biomolecules
• Microspheres have a maximum emission at 700 nm and can be excited with either 488 nm or 633 nm lasers
• Emission can be collected in either the PE-Cy5 or APC channels
• Since there is little or no emission in the FITC and PE channels, probes utilizing either of these dyes can be effectively used as reporters

Open platform microspheres for suspension array analysis in flow cytometry