Invitrogen™

NuPAGE™ Large Protein Blotting Kit

Catalog number: LP0001
Invitrogen™

NuPAGE™ Large Protein Blotting Kit

Catalog number: LP0001
Catalog Number
LP0001
Unit Size
1 kit
Price (USD)
Price: 1,204.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
LP00011 kit
Price: 1,204.00
Your price:
-
Product Overview
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The HiMark™ Pre-Stained and Unstained High Molecular Weight Protein Standards are specifically designed for large protein analysis on NuPAGE™ Novex Tris-Acetate Gels under denaturing conditions. Both standards offer a time-saving load-and-go format.

The HiMark™ Pre-stained Standard includes one pink colored orientation band and eight blue bands ranging in apparent molecular weight of 31 - 460 kDa* in NuPAGE™ Tris-Acetate Gel/SDS buffer system (Figure 1). It's suitable for monitoring electrophoresis, western transfer, and approximating molecular weight of unknown big proteins.

The HiMark Unstained Standard, along with the NuPAGE™ Tris-Acetate Gel/SDS buffer system, offers the highest accuracy for molecular weight estimation of large proteins. It consists of nine proteins, ranging in size from 40 to 500 kDa in NuPAGE™ Novex 3-8% and 7% Tris-Acetate Gel/SDS buffer systems (Figure 2). Visualize with Coomassie™ stain, silver, and fluorescent protein stains post-electrophoresis. A downloadable molecular weight calculator is designed for easy and accurate MW estimation of large proteins using the HiMark™ Unstained Standard on a NuPAGE™ Tris-Acetate Gel.**

Several NuPAGE™ Large Protein Starter Kits are available to provide you with all necessary reagents and optimized protocols for highresolution large protein separation and analysis (Table 1). Each kit includes two boxes of NuPAGE™ 3-8% Tris-Acetate Gels, Tris-Acetate SDS Buffer Kit, a staining reagent or blotting membranes, and a vial of the appropriate HiMark™ Standard.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
XCell SureLock Mini-Cell
Gel Percentage
3 to 8%
Gel Size
Mini
Gel Type
Tris-Acetate
Product Type
Protein Blotting Kit
Sample Loading Volume
25 μL
Separation Range
40 to 500 kDa
Stain Type
Unstained
Wells
10-well
Product Line
NuPAGE™
Quantity
20 gels
Thickness
1.0 mm
Well Design
1D Well

Contents & Storage

See product manual for kit contents and component storage conditions. Components of each kit are also available individually.

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