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Catalog Number
V803001
also known as V8030-01
Unit Size
1 kit
Price (USD)
Price: 1,150.00
Your price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| V803001 also known as V8030-01 | 1 kit | Price: 1,150.00 Your price: | - |
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pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five™ insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
OplE2
Product Type
Insect Cell Expression Vector
Protein Tag Position (to your gene)
C-terminal
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pMIB
Product Line
InsectSelect™
Contents & Storage
The pMIB/V5-His Vector Kit contains 20 μg each of supercoiled, lyophilized pMIB/V5-His A, B, and C vectors, pMIB/V5-His/CAT control vector, and 2 μg each of the OpIE2 Forward and Reverse Sequencing primers. Store at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
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