Super AquaBlue ELISA Substrate
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Invitrogen™

Super AquaBlue ELISA Substrate

Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signalRead more
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Catalog NumberQuantity
00-4203-56100 mL
00-4203-58500 mL
Catalog number 00-4203-56
Price (EUR)
82,00
Each
Add to cart
Quantity:
100 mL
Price (EUR)
82,00
Each
Add to cart
Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use. Like conventional ABTS substrate solutions, our new Super AquaBlue ELISA Substrate is oxidized by horse-radish peroxidase (HRP) - labeled probes resulting in a soluble, blue-green colored end product, whose absorption is measurable by spectrophotometer at 405 nm.

Unlike other ABTS solutions, Super AquaBlue ELISA Substrate is highly sensitive, extremely stable (18 months at room temperature or 24 months at 4°C). It is engineered for convenience as a single component (no additional H2O2 or other components are necessary).

Super AquaBlue ELISA Substrate is suitable for endpoint reactions, but is also ideal for kinetic assays; stopping the reaction with acid stop solution does not change the color or absorption properties of the blue-green end product.

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric
Substrate PropertiesChemical Substrate
Substrate TypeHRP (Horseradish Peroxidase)
Product TypeELISA Substrate
Quantity100 mL
Unit SizeEach

Frequently asked questions (FAQs)

What are the differences between TMB and Super AquaBlue?

TMB is a faster developing, stronger substrate that yields greater amplification/sensitivity/background. The use of an acid stop solution produces a yellow end product read at 450nm. Super AquaBlue is a slower developing blue/green substrate particularly useful for kinetic studies.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What other reagents will I need to run an ELISA using the antibody pairs?

When working with our matched pairs or uncoated ELISA kits, please refer to the user guide provided with your antibody-matched pair for kit-specific guidance. When designing your own ELISA, please utilize our ELISA Builder tool for reagent selection as well as our ELISA Development and Optimization pages for futher guidance.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.