Our new Super AquaBlue ELISA Substrate is an enhanced ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)) substrate solution for ELISA which provides high signal and sensitivity, low background. It is safe, stable, and extremely convenient to use. Like conventional ABTS substrate solutions, our new Super AquaBlue ELISA Substrate is oxidized by horse-radish peroxidase (HRP) - labeled probes resulting in a soluble, blue-green colored end product, whose absorption is measurable by spectrophotometer at 405 nm.

Unlike other ABTS solutions, Super AquaBlue ELISA Substrate is highly sensitive, extremely stable (18 months at room temperature or 24 months at 4°C). It is engineered for convenience as a single component (no additional H2O2 or other components are necessary).

Super AquaBlue ELISA Substrate is suitable for endpoint reactions, but is also ideal for kinetic assays; stopping the reaction with acid stop solution does not change the color or absorption properties of the blue-green end product.

Reported Application
ELISA