eBioscience™ Flow Cytometry Staining Buffer, 600 mL - FAQs

View additional product information for eBioscience™ Flow Cytometry Staining Buffer - FAQs (00-4222-57, 00-4222-26)

2 product FAQs found

Is it necessary to wash OneComp eBeads after staining?

One wash with 2 mL of Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) is recommended for ideal staining of OneComp eBeads. However, it may be possible in some cases to use unwashed OneComp eBeads, if the antibody staining concentration is low enough. In testing, it appears that concentrations starting below 0.06 µg per test may provide appropriate compensation values. Usage in this way should be confirmed in the individual antibody of interest.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can eFluor Organic fluorochromes and eVolve QDots be used in combination with other organic fluorochromes?

Yes, both the eFluor Organic Dyes and eVolve QDots are fully compatible with conventional fluorochromes and can be used in the same manner as the fluorochromes that they are replacing. We recommend the Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) as stated in the Staining Cell Surface Antigens for Flow Cytometry Protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.